What is a reason that a cut digest insert would not match up in length with a PCR insert in gel electrophoresis?

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase…

A:

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: In this scenario you have isolated a previously uncharacterized species of bacteria from a soil…

Q: After DNA fragments have been separated by gel electrophoresis, how can they be visualized?

A: Gel electrophoresis is a technique used for separating Deoxy ribonucleic acid (DNA) fragments based…

Q: HI Molecule 2 Molecule 3 Legend: Thick black line - RNA. Thin black line - DNA. Thin dashed line -…

A: The polymerase chain reaction was given in 1985 by " Kary Mullis". It is a rapid in-vitro molecular…

Q: In PCR amplification why is it important to have a negative control?

A: Introduction :- The Polymerase Chain Reaction (PCR), which is based on the idea of enzymatic…

Q: Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained…

A: The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of…

Q: What is a restriction digest? What does it mean if you were given a precut DNA?

A: Restriction enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at…

Q: Below are gel electrophoresis results for initial and nested PCR of the Shrimp Plant. Use the…

A: Electrophoresis is the process of separation of molecules on the basis of their charge/mass ratio…

Q: Why does the temperature constantly fluctuate inside the PCR machine? What is the purpose of the…

A: PCR, or Polymerase Chain Reaction, is a biological technique invented by Kary Mullis.

Q: ook at each PCR component listed below.  For each one, determine which steps(s) of the PCR reaction…

A: PCR is the polymerase chain reaction in which a particular sequence is amplified. PCR requires many…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.