
Concept explainers
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?
- There will be no colonies on the bacterial plate.

Introduction:
Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.
Answer to Problem 1VCQ
Correct answer:
The correct answer is option (b)- there will be blue colony only.
Explanation of Solution
Explanation for the correct answer:
Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.
Explanation for incorrect answers:
Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.
Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.
So, it is a wrong answer.
Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.
Hence, options (a ), (c), and (d) are incorrect.
The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).
Want to see more full solutions like this?
Chapter 17 Solutions
Biology 2e
Additional Science Textbook Solutions
Campbell Biology in Focus (2nd Edition)
Microbiology: An Introduction
Microbiology with Diseases by Body System (5th Edition)
Anatomy & Physiology (6th Edition)
Campbell Biology: Concepts & Connections (9th Edition)
Concepts of Genetics (12th Edition)
- circle a nucleotide in the imagearrow_forward"One of the symmetry breaking events in mouse gastrulation requires the amplification of Nodal on the side of the embryo opposite to the Anterior Visceral Endoderm (AVE). Describe one way by which Nodal gets amplified in this region." My understanding of this is that there are a few ways nodal is amplified though I'm not sure if this is specifically occurs on the opposite side of the AVE. 1. pronodal cleaved by protease -> active nodal 2. Nodal -> BMP4 -> Wnt-> nodal 3. Nodal-> Nodal, Fox1 binding site 4. BMP4 on outside-> nodal Are all of these occuring opposite to AVE?arrow_forwardIf four babies are born on a given day What is the chance all four will be girls? Use genetics lawsarrow_forward
- Explain each punnet square results (genotypes and probabilities)arrow_forwardGive the terminal regression line equation and R or R2 value: Give the x axis (name and units, if any) of the terminal line: Give the y axis (name and units, if any) of the terminal line: Give the first residual regression line equation and R or R2 value: Give the x axis (name and units, if any) of the first residual line : Give the y axis (name and units, if any) of the first residual line: Give the second residual regression line equation and R or R2 value: Give the x axis (name and units, if any) of the second residual line: Give the y axis (name and units, if any) of the second residual line: a) B1 Solution b) B2 c)hybrid rate constant (λ1) d)hybrid rate constant (λ2) e) ka f) t1/2,absorb g) t1/2, dist h) t1/2, elim i)apparent central compartment volume (V1,app) j) total AUC (short cut method) k) apparent volume of distribution based on AUC (VAUC,app) l)apparent clearance (CLapp) m) absolute bioavailability of oral route (need AUCiv…arrow_forwardYou inject morpholino oligonucleotides that inhibit the translation of follistatin, chordin, and noggin (FCN) at the 1 cell stage of a frog embryo. What is the effect on neurulation in the resulting embryo? Propose an experiment that would rescue an embryo injected with FCN morpholinos.arrow_forward
- Participants will be asked to create a meme regarding a topic relevant to the department of Geography, Geomatics, and Environmental Studies. Prompt: Using an online art style of your choice, please make a meme related to the study of Geography, Environment, or Geomatics.arrow_forwardPlekhg5 functions in bottle cell formation, and Shroom3 functions in neural plate closure, yet the phenotype of injecting mRNA of each into the animal pole of a fertilized egg is very similar. What is the phenotype, and why is the phenotype so similar? Is the phenotype going to be that there is a disruption of the formation of the neural tube for both of these because bottle cell formation is necessary for the neural plate to fold in forming the neural tube and Shroom3 is further needed to close the neural plate? So since both Plekhg5 and Shroom3 are used in forming the neural tube, injecting the mRNA will just lead to neural tube deformity?arrow_forwardWhat are some medical issues or health trends that may have a direct link to the idea of keeping fat out of diets?arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
- Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax CollegeBiology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning





