Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.

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Answer 1

Textbook Question

Chapter 17, Problem 1VCQ

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?

There will be no colonies on the bacterial

There will be blue colonies only.

There will be blue and white colonies.

The will be white colonies only.

Chapter 17, Problem 1VCQ, Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

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Students have asked these similar questions

You have two samples you have to sequence: one is a cloned plasmid that you want to verify the sequence of, and another is the cDNA library of the transcriptome of a cell. Which method would you use for each sample and why?

One of your colleagues in a molecular laboratory did not put proper labels to some of the vessels he used in his experiments. Because of that, you accidentally mixed the engineered plasmid DNA to a solution contained in a tube labelled with just "A". Apparently, that tube ("A") contains chromosomal genetic material, some RNA fragments, some glucose sugar molecules, some peptides, and long chain proteins from cell debris. You remember that the engineered plasmid will give you the chance to express a very important protein that can help cure Covid-19 patients. How do you get the plasmid from the mixed solution and bring it to its pure plasmid form (devoid of any unwanted materials for your downstream processes). Outline a step by step procedure on this and please make sure to include a step where you can prove that the content of the final vessel is indeed the engineered plasmid.

A molecular geneticist hopes to find a gene in human liver cells that codes for an important blood-clotting protein. He knows that the nucleotide sequence of a small part of the gene is CTCGACTCACA. Briefly explain how to obtain the desired gene. Briefly describe how to clone the desired gene into a cloning plasmid.

Answer 2

Textbook Question

Chapter 17, Problem 1VCQ

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?

There will be no colonies on the bacterial

There will be blue colonies only.

There will be blue and white colonies.

The will be white colonies only.

Chapter 17, Problem 1VCQ, Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

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Answer 3

Textbook Question

Chapter 17, Problem 1VCQ

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?

There will be no colonies on the bacterial

There will be blue colonies only.

There will be blue and white colonies.

The will be white colonies only.

Chapter 17, Problem 1VCQ, Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 17, Problem 1VCQ

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment?

There will be no colonies on the bacterial

There will be blue colonies only.

There will be blue and white colonies.

The will be white colonies only.

Chapter 17, Problem 1VCQ, Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

Answer 8

Expert Solution & Answer

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

Introduction:

Molecular cloning is a process to reproduce desired fragments of the genome. In cloning the plasmid molecules can be used to provide a folder in which desired genomic fragment is inserted. Then plasmids are introduced into a bacterial host. Plasmids with foreign DNA into them are called recombinant DNA molecules.

Answer 12

Answer to Problem 1VCQ

Correct answer:

The correct answer is option (b)- there will be blue colony only.

Answer 13

Explanation of Solution

Explanation for the correct answer:

Option (b) is given as ‘there will be blue colony only’. In this experiment, when the cloning vector plasmid with degraded foreign DNA is transformed into a bacterial host strain, releases a dye which turns the colony blue. Therefore, option (b) is correct.

Explanation for incorrect answers:

Option (a) is given as ‘there will be no colonies on the bacterial plate’. The colonies will be present on the plate. So, it is a wrong answer.

Option (c) is given as ‘there will be blue and white colonies no colonies’. Both blue and white colonies are only possible when plasmids are with foreign DNA insert and without insert.

So, it is a wrong answer.

Option (d) given as ‘there will be white colony only’. White colonies are not possible as plasmids with insert are not capable to express their characters. So, it is a wrong answer.

Hence, options (a ), (c), and (d) are incorrect.

Answer 14

Conclusion

The molecular cloning is a process to reproduce the desired fragments of the genome is reproduced by molecular cloning with the help of plasmid which provides a folder in which desired genomic fragment is inserted. Hence, the correct answer is option (b).

Answer 15

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