Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Chapter 17, Problem 14RQ
The chain termination method of sequencing
- uses labeled ddNITPs
- uses only dideoxynucleotides
- uses only deoxynucleotides
- uses labeled dNTPs
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Students have asked these similar questions
A Sanger product of the sequencing of a template DNA is presented
+ddTTP
+ddATP
+ddCTP
+ddGTP
Mononucleotide Pentanucleotide Trinucleotide
Dinucleotide
11-nucleotide
Hexanucleotide Octanucleotide Tetranucleotide
16-nucleotide
Decanucleotide Nonanucleotide Heptanucleotide
17-nucleotide
15-nucleotide
13-nucleotide
12-nucleotide
18-nucleotide
20-nucleotide
14-nucleotide
19-nucleotide
21-nucleotide
1. Determine the sequence of template DNA
2. Determine the mRNA sequence from this template DNA
3. Determine the the sequence of protein product derived from that DNA
What is the DNA template of the following DNA coding:
ATGGCTAACCTTGTA
Order the steps required to sequence a region of DNA using dideoxy sequencing.
Amplify the region of DNA to be sequenced
add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase
a primer binds to the single-stranded DNA template
DNA polymerase extends the primer, incorporating deoxynucleotides
a labeled dideoxynucleotide terminates the growing DNA chain
gel electrophoresis separates the mixture of DNA fragments by size
The DNA sequence is determined
denature the double-stranded DNA
Answer Bank
Chapter 17 Solutions
Biology 2e
Ch. 17 - Figure 17.7 You are working in a molecular biology...Ch. 17 - Figure 17.8 Do you think Dolly was a Finn-Dorset...Ch. 17 - Figure 17.15 In 2011, the United States...Ch. 17 - GMOs are created by. generating genomic DNA...Ch. 17 - Gene therapy can be used to introduce foreign DNA...Ch. 17 - Insulin produced by molecular cloning is of pig...Ch. 17 - Bt toxin is considered to be. a gene for modifying...Ch. 17 - The Flavr Savr Tomato: is a variety of...Ch. 17 - generated after a cDNA library is made unique...Ch. 17 - Linkage analysis. is used to create a physical map...
Ch. 17 - Genetic recombination occurs by which process?...Ch. 17 - Individual genetic maps in a given species are:...Ch. 17 - Information obtained by microscopic analysis of...Ch. 17 - The chain termination method of sequencing uses...Ch. 17 - Whole-genome sequencing can be used for advances...Ch. 17 - Sequencing an individual person’s genome is...Ch. 17 - What is the most challenging issue facing genome...Ch. 17 - Genomics can be used in agriculture to generate...Ch. 17 - Genomics cam be used on a personal level to:...Ch. 17 - What is a biomarker? the color coding of different...Ch. 17 - A protein signature is: the path followed by a...Ch. 17 - Describe the precess of Southern blottingCh. 17 - A researcher wants to study cancer cells from a...Ch. 17 - How would a scientist introduce a gene for...Ch. 17 - If you had a chance to get your genome sequenced,...Ch. 17 - Why is so much effort being poured into genome...Ch. 17 - How could a genetic map of the human genome help...Ch. 17 - Explain why metagenomics is probably the most...Ch. 17 - How can genomics be used to predict disease risk...Ch. 17 - How has proteomics been used in cancer detection...Ch. 17 - What is personalized medicine?
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- Use the gel image below to determine the sequence of the original DNA template strand sequenced using Sanger sequencing from the 5' end to the 3' end. C G T TAC GGT CAT TAC TGG CAT ATG CCA GTA ATG ACC GTA TAC GGT TACarrow_forwardDraw the gel electrophoretic pattern that would be seen in dideoxy sequenceanalysis of the DNA moleculearrow_forwardPlace the steps of base excision repair in order. Not all choices will be used. hydrolyze base First step recognize incorrect base separate strands using helicase hydrolyze incorrect nucleotide using endonuclease fill in gap with DNA polymerase close DNA ends with DNA ligase Last step Answer Bank hydrolyze abasic nucleotide using endonucleasearrow_forward
- Choose the right combination of components required to set up a polymerase chain reaction from the following: Template RNA, two primers, NTPs, and DNA polymerase Template DNA, two primers, dNTPs, and DNA ligase Template DNA, two primers, NTPs, and DNA ligase Template DNA, two primers, dNTPs, and DNA polymerasearrow_forwardWhich of the following functions are associated with the E. coli RNA polymerase? Select all that apply Helicase activity Topoisomerase activity 3' to 5' polymerase activity 5' to 3' polymerase activityarrow_forwardTranscribe an RNA from the following DNA Template sequence:5’-CCA ATG GCA ATG ATT-3’arrow_forward
- Which of the following sequences, in the double-stranded form, can probably be recognized by a restriction enzyme? Choose an answer from below? TTCCTT CGACGA GTCGAC GGTTGG GAGGACarrow_forwardEnzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonucleasearrow_forwardFrom the following DNA template, which sequence is synthesized by RNA Polymerase? 5’- T – C – C – T – A – A – G – A –3’ Group of answer choices 5’- A – G – G – A – T – T – C – T –3’ 5’- U – C – C – U – A – A – G – A –3’ 3’- A – G – G – A – U – U – C – U –5’ 3’- T – C – C – T – A – A – G – A –5’ 3’- T – G – G – A – T – T – C – T –5’arrow_forward
- Following PCR amplification of a disease gene, you perform sequence analysis using a primer 20 nuclear tides in length. partial results are depicted on the gel below which of the following are true?arrow_forwardWhich of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardTranscribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’arrow_forward
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