Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 17, Problem 13RQ
Information obtained by microscopic analysis of stained chromosomes is used in:
- radiation hybrid mapping
- sequence mapping
- RFLP mapping
- cytogenetic mapping
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Describe about fluorescence in situ hybridization (FISH).
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Chapter 17 Solutions
Biology 2e
Ch. 17 - Figure 17.7 You are working in a molecular biology...Ch. 17 - Figure 17.8 Do you think Dolly was a Finn-Dorset...Ch. 17 - Figure 17.15 In 2011, the United States...Ch. 17 - GMOs are created by. generating genomic DNA...Ch. 17 - Gene therapy can be used to introduce foreign DNA...Ch. 17 - Insulin produced by molecular cloning is of pig...Ch. 17 - Bt toxin is considered to be. a gene for modifying...Ch. 17 - The Flavr Savr Tomato: is a variety of...Ch. 17 - generated after a cDNA library is made unique...Ch. 17 - Linkage analysis. is used to create a physical map...
Ch. 17 - Genetic recombination occurs by which process?...Ch. 17 - Individual genetic maps in a given species are:...Ch. 17 - Information obtained by microscopic analysis of...Ch. 17 - The chain termination method of sequencing uses...Ch. 17 - Whole-genome sequencing can be used for advances...Ch. 17 - Sequencing an individual person’s genome is...Ch. 17 - What is the most challenging issue facing genome...Ch. 17 - Genomics can be used in agriculture to generate...Ch. 17 - Genomics cam be used on a personal level to:...Ch. 17 - What is a biomarker? the color coding of different...Ch. 17 - A protein signature is: the path followed by a...Ch. 17 - Describe the precess of Southern blottingCh. 17 - A researcher wants to study cancer cells from a...Ch. 17 - How would a scientist introduce a gene for...Ch. 17 - If you had a chance to get your genome sequenced,...Ch. 17 - Why is so much effort being poured into genome...Ch. 17 - How could a genetic map of the human genome help...Ch. 17 - Explain why metagenomics is probably the most...Ch. 17 - How can genomics be used to predict disease risk...Ch. 17 - How has proteomics been used in cancer detection...Ch. 17 - What is personalized medicine?
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- What type of label is used for fluorescence in situ hybridization (FISH) probes?arrow_forwardWhat type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experimentarrow_forwardThe figure shown describes the technique of FISH. Why is it necessaryto fix the cells (and the chromosomes inside of them) to the slides?What does it mean to fix them? Why is it necessary to denature thechromosomal DNA?arrow_forward
- Complete the tablearrow_forwardMatch the item to its best description or use. Agarose 1. produces DNA fragments RFLP 2. important tools; used to load samples into electrophoresis gels 3. small circular chromosomes in Comb bacteria 4. region of DNA that produces Micropipette different band sizes when cut with restriction enzymes Restriction Enzyme 5. produces wells in the gel where samples are added gel chamber 6. container where gel is placed to Stain or dye 8. Plasmid DNA introduce the electrical current 7. used to produce the actual gel used to visualize the otherwise colorless DNA bandsarrow_forwardFor which of the following would not require a nucleic acid probe?a. locating a gene on a chromosomeb. developing a Southern blotc. identifying a microorganism with FISHd. constructing a recombinant plasmidarrow_forward
- Why is fluorescent in situ hybridization (FISH) a useful tool for identifying micrboes? DNA is run over a chip, allowing many different gene to be tested Different probes can show spatial relationships in the cell with other bound probes None of the other choices Probes run across a membrane is a natural follow-up to gel electrophoresis Provides very detailed information about the genetic sequence of the organismarrow_forwardFor each of the following, give a brief description of the purpose and give an application Restriction enzymes Plasmids Gel Electrophoresis PCRarrow_forwardOrder the following technical steps that are required to identify a bacterium by PCR and sequencing of the 16S rRNA gene. [1 mark] - Carry out thermal cycling (amplification) of the PCR - Checking the PCR product (amplication) by agarose gel electrophoresis - Purify the PCR product - Perform a DNA extraction - Sequence the PCR product(Sanger sequencing) - Grow a fresh overnigh liquid culture - Obtain a pure bacterial culture - Set up a 16S Rrna gene PCR - Perform bioinformatic analysis (BLAST)arrow_forward
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