Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Textbook Question
Chapter 17, Problem 17RQ
What is the most challenging issue facing genome sequencing?
- the inability to develop fast and accurate sequencing techniques
- the ethics of using information from genomes at the individual level
- the availability and stability of DNA
- all of the above
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Students have asked these similar questions
Which of the following is TRUE of next generation sequencing technology?
It is required for CHIP-seq and RNA-seq
It can be used to directly sequence RNA
It has facilitated an explosion of publicly available genome sequences for eukaryotes and prokaryotes
It is extremely expensive as compared to previous sequencing approaches
Illumina is a next generation sequencing platform
It can be used to sequence proteins (proteomics)
The question is in the image along with the choices
Which of the following most accurately describes the process of DNA cloning?
set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes
set of laboratory procedures that uses living cells to mass-produce specific DNA fragments
set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip
the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology
set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid
Chapter 17 Solutions
Biology 2e
Ch. 17 - Figure 17.7 You are working in a molecular biology...Ch. 17 - Figure 17.8 Do you think Dolly was a Finn-Dorset...Ch. 17 - Figure 17.15 In 2011, the United States...Ch. 17 - GMOs are created by. generating genomic DNA...Ch. 17 - Gene therapy can be used to introduce foreign DNA...Ch. 17 - Insulin produced by molecular cloning is of pig...Ch. 17 - Bt toxin is considered to be. a gene for modifying...Ch. 17 - The Flavr Savr Tomato: is a variety of...Ch. 17 - generated after a cDNA library is made unique...Ch. 17 - Linkage analysis. is used to create a physical map...
Ch. 17 - Genetic recombination occurs by which process?...Ch. 17 - Individual genetic maps in a given species are:...Ch. 17 - Information obtained by microscopic analysis of...Ch. 17 - The chain termination method of sequencing uses...Ch. 17 - Whole-genome sequencing can be used for advances...Ch. 17 - Sequencing an individual person’s genome is...Ch. 17 - What is the most challenging issue facing genome...Ch. 17 - Genomics can be used in agriculture to generate...Ch. 17 - Genomics cam be used on a personal level to:...Ch. 17 - What is a biomarker? the color coding of different...Ch. 17 - A protein signature is: the path followed by a...Ch. 17 - Describe the precess of Southern blottingCh. 17 - A researcher wants to study cancer cells from a...Ch. 17 - How would a scientist introduce a gene for...Ch. 17 - If you had a chance to get your genome sequenced,...Ch. 17 - Why is so much effort being poured into genome...Ch. 17 - How could a genetic map of the human genome help...Ch. 17 - Explain why metagenomics is probably the most...Ch. 17 - How can genomics be used to predict disease risk...Ch. 17 - How has proteomics been used in cancer detection...Ch. 17 - What is personalized medicine?
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- What molecular biology technique is used to amplify DNA from an evidence sample? Reverse dot blotting Southern hybridization Cloning Polymerase chain reaction (PCR) Capillary electrophoresisarrow_forwardHumans have very similar DNA sequences, with approximately 999/1000 letters being ide One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more peoplearrow_forwardWhich of the following is correct regarding sequencing by synthesis? All four nucleotides are added simultaneously during the sequencing phase. All four nucleotides have fluorescent tags. Homopolymer error rates are significantly lower than other methods of second-generation sequencing. All of the abovearrow_forward
- What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic site? Sequencing of the DNA by Sanger Northern blotting Southern blotting RFLP analysis in agarose gel electrophoresis PCRarrow_forwardGenetic Engineering Terms Name: Draw a line to connect each pair of boxes DNA sequencing The use of an organism, or a component of an organism or other biological system, to make a product or process. DNA cloning The sequencing, analysis, and cutting-and-pasting of DNA A technique to make many copies of a specific DNA region in vitro (in a test tube rather than anorganism) Recombinant DNA Polymerase chain reaction A technique used.to separatę DNA fragments according to their size Gel electrophoresis DNA that is assembled out of fragments from multiple sources Biotechnology A molecular biology technique that makes many identical copies of a piece of DNA, such as a gene DNA technology The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA. ww tİ6111749/enetic-engineering-temsarrow_forwardWhat is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the abovearrow_forward
- Whole-genome sequencing can be used for advances in: the medical field agriculture biofuels all of the abovearrow_forwardHow can we ensure that a patient’s privacy is maintained as genome information accumulates within medical records? How would you feel about allowing your genome sequence to be available for use in research?arrow_forwardWhich of the following is not true about microarrays? Group of answer choices A microarray is a glass slide with single-stranded DNA attached to it. A microarray is covered in spots, where each spot represents a different sequence that will be assessed in the experiment. A microarray can assess hundreds of thousands of variants simultaneously. The same microarray slide can be used either for DNA microarrays or RNA microarraysarrow_forward
- Choose the single most appropriate description of how most Next Generation sequencing methods work. Template DNA is attached to 'chips', free di-nucleotides are added to synthesise new DNA, computers read the results. Template DNA is attached to 'chips', di-deoxy nucleotides are added to synthesise new DNA, computers read the results. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. They are bound to a membrane and sequenced using lasers. Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. The fragments are then bound to a membrane and sequenced using light emission as the reporter. Sequencing by synthesis of short template fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes. Sanger sequencing of short fragments, using various 'reporters' and computing power to…arrow_forwardWhich of the following is NOT an advantage of the PCR method over traditional cloning methods for producing desired DNA sequences? PCR is a partially automated technique so less prone to human error PCR uses DNA polymerase to generate new DNA sequences PCR is much faster than traditional methods of cloning DNA in order to amplify it PCR does not require the production of recombinant organisms to amplify the DNAarrow_forwardWhich of the following statements is true about consensus sequences in DNA? Group of answer choices a consensus sequence is a technology for next-generation genomics consensus sequences are found in prokaryotes but not eukaryotes consensus sequences are considered 'junk DNA' with little biological relevance a consensus sequence reports the most common bases found in a specific nucleotide sequencearrow_forward
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