Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Textbook Question
Chapter 13, Problem 10QP
Cloning Genes Is a Multistep Process
Which enzyme is responsible for covalently linking DNA strands together?
- a. DNA polymerase
- b. DNA ligase
- c. EcoRl
- d. restriction enzymes
- e. RNA polymerase
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A scientist randomly mutates the DNA of a bacterium. She then sequences the bacterium’s daughter cells, and finds that the daughters have many errors in their replicated DNA. The parent bacterium likely acquired a mutation in which enzyme? a. DNA ligase b. DNA pol II c. Primase d. DNA pol I
Which of these is a polymer made of nucleotides?
a.
RNA polymerase
b.
CAS9
c.
restriction enzyme
d.
mRNA
What is used to cut the DNA in panels 1 and 2?
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Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward
- PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardWhy do prokaryotes produce restriction enzymes? A. Restriction enzymes are produced to enable bacteria to repair lesions in its DNA B. Restriction enzymes enable prokaryotes to assimilate genes or fragments of DNA from its environment C. Restriction enzymes excise phage DNA from prokaryotes thereby protecting them from lysis. D. Restriction enzymes help unwind DNA and facilitate DNA replication in prokaryotes E. Restriction enzymes are necessary for editing DNA during replication.arrow_forwardWhich of the following is not a part of the Sanger method tosequence DNA?a. dideoxynucleotides b. DNA polymerase c. electrophoresis d. reverse transcriptasearrow_forward
- What will most likely be the effect of the change in the DNA molecule? * A. the change will cause a harmful mutation B. the DNA molecule will be unable to replicate C. the organism will be reproduce D. the DNA molecules will code for a different protein Original strand: T A C G T A T G A A G C, Mutant strand: T A G C T A T G A A G C. what type of mutation? * A. Substitution B. Deletion C. Insertion D. Nonsense Which mechanism contributes to accuracy during DNA Replication? * A. The mismatch repair system recognizes an incorrect base-pair and corrects the mistake in the non-methylated strand B. Using primers increase accuracy because the first nucleotides is a new nucleic acid chain are more like to be correct. C. all DNA polymerase have a 5'-3' exonuclease activity which can remove…arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardDNA polymerases that corrects errors and mismatches in DNA is called? A. Post replication repair B. Base excision repair C. Nucleotide excision repair D. Proofreadingarrow_forward
- Which enzyme adds RNA nucleotides to mark the starting point for the synthesis of a new DNA strand? A. DNA polymerase I B. DNA polymerase III C. primase D. helicase The spontaneous loss of amino groups from adenine in dna results in hypoxanthine an uncommon base opposite thymine. what combination of proteins could repair such damage? A. telomerase, primase, DNA polymerase B. nuclease, DNA polymerase, DNA ligase C. telomerase, helicase, single-strand binding protein D. DNA ligase, replication fork proteins, adenylyl cyclase A section of DNA has the following sequence of nitrogenous bases: CGATTACAG. Which of the following sequence would be produced as a result of transcription? A. CGTUUTCTG B. GCTAATGTC C. CGAUUACAG D. GUAAUGUCarrow_forwardWhich of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translationarrow_forwardIf a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010arrow_forward
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