Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 14QP
Summary Introduction
To describe: The type of vector used in the preparation of a cloned genomic library from human tissue culture cell line.
Introduction: In the manufacturing of human genomic library, the fragments of DNA used in the process can vary in size which can be picked up by the plasmid easily. About 8 million plasmids are required to include all the genetic information from an individual cell.
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Transcriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment.
(1) DNA sequencing
(2) Allow for hybridization and wash excess cRNA.
(3) Mix labeled cRNA with array chip.
(4) PCR amplification
(5) Measure fluorescence intensity to determine abundance of transcripts.
(6) Add labeled cRNA at each microarray location.
(7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts.
(8) mRNA isolation from cells
(9) Prepare fluorescently labeled cRNA probes
(10) cDNA synthesis
What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the genome sequence.
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?arrow_forwardoptions to choose from: - black - the correct answer is not listed - red - yellow or black - red or green - yellow - green - all of thesearrow_forwardPrimer Designing:arrow_forward
- What is an advantage of making a cDNA library rather than a genomic library?arrow_forwardExperiment A microarray was hybridized with a mixture of two differentially labeled fluorescent cDNAs, one prepared using retinal RNA of 1-day-old mice (labeled with a green fluorescent dye) and the other prepared using retinal RNA of 28-day-old mice (red fluorescence). The two probes were prepared from identical amounts of retinal tissues and were mixed together for hybridization to the microarray. Unhybridized probes were washed away, and the microarray was photographed in a fluorescence microscope.arrow_forwardWhat are the following features that are required facilitate cloning into a vector.arrow_forward
- You want to make a library with DNA fragments averagingover 100 kb in length. Which vectors discussed in this chapter would be most appropriate to use? Why?arrow_forwardIn cloning a specific fragment from a mixture of different fragments of DNA, three classes of plasmids can be produced: vector containing the desired fragment (gene of interest), vector containing other fragments, and re- ligated vector containing no inserted DNA. What class of vector would you expect to find at the highest frequency? Multiple Choice All three types of vector will be found in approximately equal proportions Vector containing random genomic DNA fragments Vector containing the gene of interest Vector with no insert wwarrow_forwardYou need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR productsarrow_forward
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