Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 13QP
Summary Introduction
Introduction: In the formation of human genomic library, the fragments of DNA used in the process can vary in size which can be picked up by the plasmid easily. About 8 million plasmids are required to include all the genetic information from an individual cell.
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What is a genomic DNA library?
O A. All DNA fragments identified with a probe
O B. A general collection of all genes sequenced thus far
C. A DNA fragment inserted into a vector
O D. A collection of DNA fragments that make up the entire genome of a particular organism that have been cloned into a vector
O E. A collection of DNA fragments that make up the entire genome of a particular organism
O e. Gene amplification
CLEAR MY CHOICE
To study the function of any gene of interest you would perform the loss and gain of function approaches
by either deleting or re-expressing the gene of interest, which of the following can be used to determine
and quantify the activity of the gene?
Oa.
Western blotting
O b. Gene knockdown
O. PCR/OLA
O d. Microscopy
O e. DNA hybridization
Paternity testing can be detected most precisely by using technique
In next-generation sequencing genomes are sequenced by.
breakdown, ligated with a
the
(random/selective) DNA
(common/unique) oligonucleotide, attached to the surface via
(3'/5') end.
Select one:
a. selective, unique, 3'
b. selective, unique, 5'
c. random, common, 5'
d. selective, common,
3'
e. random, unique, 5'
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forwardMatch Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v Elucidation of patterns of coordinate gene regulation A. Recombinant DNA Technology B. Sanger sequencing |derives its power from the distinctive evolutionary patterns C. Restriction Enzymes and Ligase v involves joining a donor DNA fragment of interest to a vector D. GRNA of CRISPR technology Enzymes that cleave the DNA strands E. Restriction enzymes Enzymes used in Recombinant DNA Technology F. Functional Genomics homologous to target DNA that can base pair with it The first sequencing technology that uses amplification and fluorescent dyearrow_forwardA has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forward
- = Menu #2 Mol Bio Mutation QU... X + Create All tools Edit Convert E-Sign Q Search Complete the following tasks. Sign in Find text or tools Q H You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these…arrow_forwardCheck all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. B. Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons. C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. D. The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.arrow_forwardHow the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?arrow_forward
- H2. Please explain with details also explain wrong optionsarrow_forwardMatch each of the terms in the left column to the bestfitting phrase from the right column.a. exome 1. a discrete part of a protein that provides a unitof functionb. de novo gene 2. a nonfunctional member of a gene familyc. gene desert 3. the joining together of exons in a gene indifferent combinationsd. pseudogene 4. most frequent residues, either nucleotide oramino acid, found at each position in asequence alignmente. syntenic block 5. set of genes related by processes ofduplication and divergencef. orthologs 6. chromosomal region with the same genes inthe same order in two different speciesg. naturalselection7. genes with sequence similarities in twodifferent species that arose from a commonancestral geneh. consensussequence8. genes that arose by duplication within aspeciesi. gene family 9. genomic DNA sequences containing exonsj. paralogs 10. gene-poor region of the genomek. alternativeRNA splicing11. recently evolved from intergenic DNAsequencesl. protein domain 12. progressive…arrow_forwardThe BLAST program is a tool fora. inserting many DNA fragments into a cell at the same time.b. translating a DNA sequence into an amino acid sequence.c. identifying homology between a selected sequence and geneticsequences in databases.d. all of the above.e. both b and c.arrow_forward
- A. What are some reasons a person might want to clone a human? B. What animal was cloned in 1885?arrow_forwardCRISPR sequences are commonly found in O a. Prokaryotes; DNAse O b. Eukaryotes; Cas9 Ok. Eukaryotes; DNAse O d. Prokaryotes; Hexokinase O e. Prokaryotes; Cas9 and, when associated with an enzyme known as can serve to edit genes within an organism.arrow_forwardHi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?arrow_forward
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