Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 13QP
Summary Introduction
Introduction: In the formation of human genomic library, the fragments of DNA used in the process can vary in size which can be picked up by the plasmid easily. About 8 million plasmids are required to include all the genetic information from an individual cell.
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What is a genomic DNA library?
O A. All DNA fragments identified with a probe
O B. A general collection of all genes sequenced thus far
C. A DNA fragment inserted into a vector
O D. A collection of DNA fragments that make up the entire genome of a particular organism that have been cloned into a vector
O E. A collection of DNA fragments that make up the entire genome of a particular organism
O e. Gene amplification
CLEAR MY CHOICE
To study the function of any gene of interest you would perform the loss and gain of function approaches
by either deleting or re-expressing the gene of interest, which of the following can be used to determine
and quantify the activity of the gene?
Oa.
Western blotting
O b. Gene knockdown
O. PCR/OLA
O d. Microscopy
O e. DNA hybridization
Paternity testing can be detected most precisely by using technique
In next-generation sequencing genomes are sequenced by.
breakdown, ligated with a
the
(random/selective) DNA
(common/unique) oligonucleotide, attached to the surface via
(3'/5') end.
Select one:
a. selective, unique, 3'
b. selective, unique, 5'
c. random, common, 5'
d. selective, common,
3'
e. random, unique, 5'
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- A has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forward= Menu #2 Mol Bio Mutation QU... X + Create All tools Edit Convert E-Sign Q Search Complete the following tasks. Sign in Find text or tools Q H You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these…arrow_forwardHow the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?arrow_forward
- H2. Please explain with details also explain wrong optionsarrow_forwardThe BLAST program is a tool fora. inserting many DNA fragments into a cell at the same time.b. translating a DNA sequence into an amino acid sequence.c. identifying homology between a selected sequence and geneticsequences in databases.d. all of the above.e. both b and c.arrow_forwardCRISPR sequences are commonly found in O a. Prokaryotes; DNAse O b. Eukaryotes; Cas9 Ok. Eukaryotes; DNAse O d. Prokaryotes; Hexokinase O e. Prokaryotes; Cas9 and, when associated with an enzyme known as can serve to edit genes within an organism.arrow_forward
- Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?arrow_forwardAn organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. 0.000 de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genomearrow_forwardShort tandem repeats (STR) profiling is based on O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones. O B. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers. OD. the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person.arrow_forward
- PCR errors during library amplification are one possible source of false positive results. If an error occurs in the first round of amplification, all the subsequent copies of that library fragment will also carry the variant, even though it is not present in the genome. However, reads from other library fragments spanning this same region will not have the variant, which means that identifying PCR copies, or clones, of library fragments during analysis can help to identify these types of errors. Based on what you know about PCR, which of the following statements would be true about the PCR clones in a sequencing library? A. PCR is subject to amplification bias, so reads derived from PCR clones will only map to regions that are not GC-rich. B. PCR is subject to amplification bias, so reads derived from PCR clones will only map to non-repetitive regions. C. PCR produces identical copies, so reads derived from PCR clones would map to the exact same location. D. PCR introduces many…arrow_forwardanatomical distribution of gene expression can assessedby...... in situ hybridization rnai nortbern blotting genOmic pcrarrow_forwardExplain why DNA ladders are usually included during gel electrophoresis. One aspect of PCR that can be modified is the annealing temperature. In general, higher annealing temperatures show more specificity towards a single template, whereas lower annealing temperatures show less specificity and may bind to multiple regions throughout the genome. Discuss how using an annealing temperature that is too high or too low might influence the results of a PCR assay (and gel electrophoresis results) such as the one used in this study.arrow_forward
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