Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 12QP
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To describe: The steps of cloning a segment of DNA.
Introduction: The
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Gene cloning is a process in which a gene of interest is identified and made into multiple copies.
Illustrate the steps in gene cloning using Escherichia coli as the host cell after PCR has been
carried out to amplify a gene of interest.
Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.
Explain why the precise length of the template DNA sequence does not become amplified until the third cycle. Draw a picture that explains your answer.
For: Electrophoresis
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardCloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwardMake a table comparing the advantages and disadvantages of PCR and gene cloning for amplifying DNA fragments.arrow_forward
- Explain what restriction enzymes are, how they function and how they can be used to make recombinant DNAarrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forwardTransgenic bacteria can be used to make an alanine rich (GM) plant. Explain how bacteria can be used to produce large amounts as a cheap source of protein. Your explanation will include the role of: Restriction enzymes, plasmids, recombinant DNA, and bacteria.arrow_forward
- See the restriction enzyme map below. The total DNA length is 1800 base pairs. If this DNA is cut using three restriction enzymes, namely Kpnl, Sall and EcoRI, it yields four fragments with sizes of 390 bR. 810 bp, 270 bp www and 330 bp. Kpnl Sall EcoRI 390 810 270 330 1800 bp 1. If you were to subject this digested DNA to agarose gel electrophoresis, what would your gel look like? Draw a detailed picture of your gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. 2. You are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exac fragment sizes are not important.arrow_forwardA restriction enzyme digests DNA into fragments.term the technique used to check the progression of this enzyme and separate DNA fragments.arrow_forwardBiotechnology There are several different tools used to study or manipulate DNA. Match the description to the technology. NOTE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice. Polymerase Chain Reaction (PCR) Replacing defective genes that caused a disease Gel Electrophoresis Circular DNA used to insert genes Molecular cloning Cuts DNA into smaller pieces Tool for gene editing Restriction enzyme Creates larger quantities of DNA Plasmids Produces genetically identical DNA fragments Separates fragments of DNA by size CRISPR Gene therapyarrow_forward
- Explain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardDescribe how you would clone a gene by positional cloning.Explain how a (previously made) contig would make this taskmuch easier.arrow_forwardState the characteristics that would be required for using a restriction endonuclease with human DNA, and explain why you needed to use the empty DNA fragment.arrow_forward
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