Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 9QP
a.
Summary Introduction
To determine: The size of bands obtained after the cutting of DNA with EcoRI.
Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
b.
Summary Introduction
To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.
Introduction:
Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
c.
Summary Introduction
To explain: The size of bands obtained after the cutting of DNA with all three enzymes.
Introduction:
Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
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On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img)
i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples?
ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?
See the restriction enzyme map below. The total DNA length is 1800 base
pairs. If this DNA is cut using three restriction enzymes, namely Kpnl, Sall
and EcoRI, it yields four fragments with sizes of 390 bR. 810 bp, 270 bp
www
and 330 bp.
Kpnl
Sall
EcoRI
390
810
270
330
1800 bp
1. If you were to subject this digested DNA to agarose gel
electrophoresis, what would your gel look like? Draw a detailed
picture of your gel. Remember to indicate the direction in which your
DNA is moving and also show any reference samples. Also
remember to show all components of your gel.
2. You are provided with coiled DNA and plasmid DNA that you subject
to gel electrophoresis. Draw this gel. Remember to indicate the
direction in which your DNA is moving and also show any reference
samples. Also remember to show all components of your gel. Exac
fragment sizes are not important.
A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion?
B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be?
C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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