Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 6QP
Summary Introduction
To describe: The reasons that the presence of restriction enzymes inside the bacterial cell does not affect bacterial chromosomes.
Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes are also termed as “restriction endonucleases.” The restriction enzyme plays an important role in the production of recombinant DNA.
Summary Introduction
To explain: The reasons behind the presence of restriction enzymes inside the bacterial cell.
Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes are also termed as “restriction endonucleases.” The restriction enzyme plays an important role in the production of recombinant DNA.
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a) what are restriction enzymes?
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Why don’t bacteria cut up their own DNA when they produce restriction enzymes?
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhat is the function of a restriction enzyme?arrow_forwardThe genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because Choose an answer from below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the restriction recognition sequences in the genome are protected by a microRNA. none of the abovearrow_forward
- A double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition sites. How many fragments will be produced? a) 2 b) 3 c) 4 d) 5arrow_forwardYou want to clone a 6,000 bp DNA fragment in E. coli. Which cloning vectors would be appropriate? How will you select transformants?arrow_forwardWhich of the following is true about restriction endonucleases?a) Type I and II requires ATP to move along DNAb) Type I, II and III requires ATP to move along DNAc) Type II requires no ATP and cleaves DNA within recognition sequenced) Type II requires ATP and cleaves DNA within recognition sequencearrow_forward
- Explain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait an important part of the recombinant DNA technology process in the biotechnology industry?arrow_forwardWhat roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?arrow_forwardBoth cloning and PCR can be used for making copies of DNA. What is the advantage or limitation of one over the other?arrow_forward
- You’re working in a research lab, and your current task is to clone the gene that codes for tyrosinase from potatoes. You grind up some potato, extracts the DNA from it and digests the DNA with two different restriction enzymes (separately, not together): EcoRI and BamHI. You then obtain the cloning vector, pUC19, and digest it with the same two enzymes. You then run a gel which is shown to the right. You mix the potato DNA (digested with the enzyme you specified in part B) with cloning vector DNA (digested with the same enzyme). You then add the mixture to E. coli cells and carry out a transformation procedure so that the cells can each update a plasmid. You then plate the cells on a plate containing antibiotic. What antibiotic would be in the plate? Why would there be antibiotic in the plate? Be specific! Unfortunately, you don’t get a single bacterial colony to grow on the plate. Not even one! You review your procedure and realize that when mixing the digested potato DNA…arrow_forwardHow do Restriction Enzymes like EcoRI work?arrow_forwardA biologist is attempting to clone the gene encoding a particular enzyme (Enz) into a plasmid vector in E.coli. This plasmid has a gene encoding a green fluorescent protein (GFP) as well as a gene for tetracycline antibiotic resistance (TetR). The restriction site (to clone foreign DNA into) is within the GFP sequence. Which of the following would be expected when trying to see which E. coli cells acquired the recombinant plasmid (i.e., carrying the Enz gene)? Bacteria UNABLE to grow on tetracycline-containing media AND are NOT able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria able to grow on tetracycline-containing media AND that are NOT able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria able to grow on tetracycline-containing media AND are able to make green fluorescent protein are the ones that contain the recombinant plasmid. Bacteria UNABLE to…arrow_forward
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