A student purified a protein, resulting in a fraction with a volume of 240 µL. The sample has a corrected A280 of 0.234, and the blank corrected A280 was 0.069. (Both values were measured with a path length of 1.00 cm.) The student then added 6.00 µL of the sample to a reaction tube. Calculate the mass of protein in the reaction (in µg).

Protein are measured by spectrophotometer. They absorb the ultraviolet radiations at specific…

Answered: A student purified a protein, resulting…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A student purified a protein, resulting in a fraction with a volume of 240 µL. The sample has a corrected A₂₈₀ of 0.234, and the blank corrected A₂₈₀ was 0.069. (Both values were measured with a path length of 1.00 cm.) The student then added 6.00 µL of the sample to a reaction tube. Calculate the mass of protein in the reaction (in µg).

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