The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear range? NOTE: it is essential that enzymatic activity is measured in the linear range, during which time the enzymatic activity is greatest and the concentration of substrate is not limiting. The decision on a suitable linear range often comes down to the judgement of the researcher. The linear range should ideally include at least three data points, but there is no limit to how many data points can be included so long as they follow a linear trend

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below.

Time (min)	Concentration (μM)
0.5	6.01
1	11.78
1.5	17.6
2	23.51
2.5	29.58
3	35.31
3.5	39.73
4	44.5
4.5	48.18
5	50.05
5.5	52.72
6	54.01
6.5	55.06
7	55.65
7.5	56.39
8	56.74
8.5	57.49
9	58.03
9.5	58.61
10	58.69

Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R² value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate.

To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear range?

NOTE: it is essential that enzymatic activity is measured
in the linear range, during which time the enzymatic activity is greatest and the concentration of
substrate is not limiting. The decision on a suitable linear range often comes down to the judgement of
the researcher. The linear range should ideally include at least three data points, but there is no limit to
how many data points can be included so long as they follow a linear trend

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