Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength, or 9.9 mL of the sample plus 0.1 mL of 10x broth (here a fluorescent Brilliant Green Broth). The 1 mL tubes are 1 mL sample plus 9 mL of normal broth (so a ten-fold dilution). The 0.1 mL are that much sample with 9.9 mL of the broth (so a hundred-fold dilution). Dark green indicates coliform presence. Use the table and the figure and give me the MPN. To do so, count the number of positive tubes at each dilution. Then match the counts to the set of values that most closely fits. Yes, you need to consider the set of values for None, 1 and 0.1 taken together. For example, 5,2,1. What is the MPN estimate for this well water sample? If safe drinking water is <5 cells/100 mL would you drink this well water?
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength, or 9.9 mL of the sample plus 0.1 mL of 10x broth (here a fluorescent Brilliant Green Broth). The 1 mL tubes are 1 mL sample plus 9 mL of normal broth (so a ten-fold dilution). The 0.1 mL are that much sample with 9.9 mL of the broth (so a hundred-fold dilution). Dark green indicates coliform presence. Use the table and the figure and give me the MPN.
To do so, count the number of positive tubes at each dilution. Then match the counts to the set of values that most closely fits. Yes, you need to consider the set of values for None, 1 and 0.1 taken together. For example, 5,2,1.
What is the MPN estimate for this well water sample?
If safe drinking water is <5 cells/100 mL would you drink this well water?
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