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Protein Solubility pll 3.5 4.5 6.5 7.5 Dilution Factor 5 5 50 50 Absorbance 0.098 0.027 0.028 0.032 Conc.(mg/ml) 0.195 0.042 0.130 0.044 0.053 % solubility 4.179% 8.9% 2.786% 9.429% 11.357% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. 3 15 7.5 5.5 5 0.065 Protein Solubility Versus pl 4.5 pll Vale The above figure shows a plot of protein solubility ver y versus pl. 8.5 50 0.054 0.100 21.429%

Protein Solubility pll 3.5 4.5 6.5 7.5 Dilution Factor 5 5 50 50 Absorbance 0.098 0.027 0.028 0.032 Conc.(mg/ml) 0.195 0.042 0.130 0.044 0.053 % solubility 4.179% 8.9% 2.786% 9.429% 11.357% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. 3 15 7.5 5.5 5 0.065 Protein Solubility Versus pl 4.5 pll Vale The above figure shows a plot of protein solubility ver y versus pl. 8.5 50 0.054 0.100 21.429%

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Protein Solubility 3.5 5 0.028 Absorbance 0.098 Conc.(mg/ml) 0.195 0.044 0.9% 2.786% 9.429% 11.3: % solubility 4.179% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Dilution Factor 30 22.5 pH 15 7.5 1.5 4.5 5 0.027 3 0.042 5.5 5 0.068 0.130 Protein Solubility Versus pH 6 6.5 4.5 pH Value The above figure shows a plot of protein solubility versus pH. 50 Please provide a brief discussion and explanation of the results. 7.5 7.5 50 0.032 0.053 9 8.5 50 0.054 0.100 21.429%
Transcribed Image Text:Protein Solubility 3.5 5 0.028 Absorbance 0.098 Conc.(mg/ml) 0.195 0.044 0.9% 2.786% 9.429% 11.3: % solubility 4.179% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Dilution Factor 30 22.5 pH 15 7.5 1.5 4.5 5 0.027 3 0.042 5.5 5 0.068 0.130 Protein Solubility Versus pH 6 6.5 4.5 pH Value The above figure shows a plot of protein solubility versus pH. 50 Please provide a brief discussion and explanation of the results. 7.5 7.5 50 0.032 0.053 9 8.5 50 0.054 0.100 21.429%
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