I need help with 2 but 1 tells you other measurements

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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I need help with 2 but 1 tells you other measurements

**Procedure:**

1. **Preparation of CuSO₄ Solution:**
   - Begin by measuring approximately 0.40 g of copper (II) sulfate (CuSO₄) to make a solution. Note that the formula refers to anhydrous CuSO₄, but if instead, a blue crystalline solid is provided, it indicates CuSO₄·5H₂O. If you're unsure, ask your instructor since the molecular weight of the solid will differ.
   - Dissolve the measured CuSO₄ in 300-400 mL of water. If the solution appears light blue-green, it is likely CuSO₄·5H₂O.

2. **Calculation of NaOH Solution Volume:**
   - Use the mass of your solid to calculate the volume of 0.20 M NaOH solution required to fully react with the CuSO₄. Adjust the calculation to ensure the copper is the limiting reactant, avoiding excess NaOH. 
   - The formula used is:

     \[
     \text{Volume of NaOH} = \frac{\text{mass of CuSO₄} \times \text{molarity of CuSO₄}}{\text{molarity of NaOH}}
     \]

   - This formula ensures you add an appropriate amount of NaOH solution.

3. **Addition of NaOH Solution:**
   - Carefully add the calculated volume of NaOH solution to the beaker containing the dissolved CuSO₄. Stir gently, then allow the solid to settle.

4. **Inspection and Adjustment:**
   - After the precipitate has settled, inspect the solution. If it is still tinted blue, it indicates some copper ions did not react. In such a case, allow it to settle further and add a few more mL of NaOH until the solution becomes clear, indicating all copper has been precipitated. If it becomes clear before adding excess NaOH, this means the reaction is complete. 

_Note: It is fine to slightly overdo the addition of NaOH to ensure complete reaction._
Transcribed Image Text:**Procedure:** 1. **Preparation of CuSO₄ Solution:** - Begin by measuring approximately 0.40 g of copper (II) sulfate (CuSO₄) to make a solution. Note that the formula refers to anhydrous CuSO₄, but if instead, a blue crystalline solid is provided, it indicates CuSO₄·5H₂O. If you're unsure, ask your instructor since the molecular weight of the solid will differ. - Dissolve the measured CuSO₄ in 300-400 mL of water. If the solution appears light blue-green, it is likely CuSO₄·5H₂O. 2. **Calculation of NaOH Solution Volume:** - Use the mass of your solid to calculate the volume of 0.20 M NaOH solution required to fully react with the CuSO₄. Adjust the calculation to ensure the copper is the limiting reactant, avoiding excess NaOH. - The formula used is: \[ \text{Volume of NaOH} = \frac{\text{mass of CuSO₄} \times \text{molarity of CuSO₄}}{\text{molarity of NaOH}} \] - This formula ensures you add an appropriate amount of NaOH solution. 3. **Addition of NaOH Solution:** - Carefully add the calculated volume of NaOH solution to the beaker containing the dissolved CuSO₄. Stir gently, then allow the solid to settle. 4. **Inspection and Adjustment:** - After the precipitate has settled, inspect the solution. If it is still tinted blue, it indicates some copper ions did not react. In such a case, allow it to settle further and add a few more mL of NaOH until the solution becomes clear, indicating all copper has been precipitated. If it becomes clear before adding excess NaOH, this means the reaction is complete. _Note: It is fine to slightly overdo the addition of NaOH to ensure complete reaction._
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