The kinetic data for the activity of 0.01mM enzyme is shown below. The concentration of {S} is millimolar and reaction rate is millimolar per second. Take this information down. Produce a Lineweaver-Burke graph using the data below, find the Km, find the Vm, and calculate the kcat for this enzyme. Turn in a single page with the graph (title, axes labels and units, etc) and the values of Km, Vm, and kcat (see the example image below)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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An enzyme reacts with substrate S to produce
P.
E +SS ES → E + P
The kinetic data for the activity of 0.01mM
enzyme is shown below. The concentration of
{S} is millimolar and reaction rate is millimolar
per second. Take this information down.
Produce a Lineweaver-Burke graph using the
data below, find the Km, find the Vm, and
calculate the kcat for this enzyme. Turn in a
single page with the graph (title, axes labels
and units, etc) and the values of Km, Vm, and
kcat (see the example image below)
Name: J Doe
Lineweaver-Burke Graph for Enzyme X
1/v
(s/mM)
1/s
(/mM)
K - ?
keat - ?
Transcribed Image Text:An enzyme reacts with substrate S to produce P. E +SS ES → E + P The kinetic data for the activity of 0.01mM enzyme is shown below. The concentration of {S} is millimolar and reaction rate is millimolar per second. Take this information down. Produce a Lineweaver-Burke graph using the data below, find the Km, find the Vm, and calculate the kcat for this enzyme. Turn in a single page with the graph (title, axes labels and units, etc) and the values of Km, Vm, and kcat (see the example image below) Name: J Doe Lineweaver-Burke Graph for Enzyme X 1/v (s/mM) 1/s (/mM) K - ? keat - ?
{S}
V
10.00
71
50.00
300
200.00
750
400.00
1000
Transcribed Image Text:{S} V 10.00 71 50.00 300 200.00 750 400.00 1000
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