A student titrated a 250 mL solution of an unknown amino acid with 1.0 M sodium hydroxide and drew the below titration curve. From this titration curve they found that the amino acid had three functional groups with the pKa of the functional groups displayed on the graph. To do this titration they added in a total of 75 mL of 1.0 M sodium hydroxide. They also calculated that they added a total of 15 mL of sodium hydroxide to get to pK₁ (indicated by arrow) and a total of 37 mL of sodium hydroxide to get to pK₂(indicated by arrow). What was the initial concentration of the amino acid solution in milimolar (mM)?
A student titrated a 250 mL solution of an unknown amino acid with 1.0 M sodium hydroxide and drew the below titration curve. From this titration curve they found that the amino acid had three functional groups with the pKa of the functional groups displayed on the graph. To do this titration they added in a total of 75 mL of 1.0 M sodium hydroxide. They also calculated that they added a total of 15 mL of sodium hydroxide to get to pK₁ (indicated by arrow) and a total of 37 mL of sodium hydroxide to get to pK₂(indicated by arrow). What was the initial concentration of the amino acid solution in milimolar (mM)?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:14
12
10
pH 8
6
4
0
Titration curve for an unknown amino acid
pk₁=1.8
pK₂=6.0
pK, 9.2
Volume (mL) of 1 M NaOH added
A student titrated a 250 mL solution of an unknown amino acid with 1.0 M sodium hydroxide and drew the below titration
curve. From this titration curve they found that the amino acid had three functional groups with the pKa of the functional
groups displayed on the graph. To do this titration they added in a total of 75 mL of 1.0 M sodium hydroxide. They also
calculated that they added a total of 15 mL of sodium hydroxide to get to pK₁ (indicated by arrow) and a total of 37 mL of
sodium hydroxide to get to pK₂(indicated by arrow).
What was the initial concentration of the amino acid solution in milimolar (mM)?
Provide the answer with NO decimal places. You do not have to indicate the unit in the answer field!
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