Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation exchange chromatography. You add your protein to the column in a solution at pH of 3 and collect the eluant as fraction A. You then wash the column with a buffer at pH 5 and collect the eluant as fraction B. You do a third wash of your column with buffer at pH of 7 and collect the eluant as fraction C. You do a fourth and final wash of the column with buffer at pH 12 and collect the eluant as fraction D. Which fraction contains most of your protein? O Fraction A O Fraction B O Fraction C O Fraction D O None of the above O All of the above

Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation exchange chromatography. You add your protein to the column in a solution at pH of 3 and collect the eluant as fraction A. You then wash the column with a buffer at pH 5 and collect the eluant as fraction B. You do a third wash of your column with buffer at pH of 7 and collect the eluant as fraction C. You do a fourth and final wash of the column with buffer at pH 12 and collect the eluant as fraction D. Which fraction contains most of your protein? O Fraction A O Fraction B O Fraction C O Fraction D O None of the above O All of the above

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

100%

Choices are:

Fraction A

Fraction B

Fraction C

Fraction D

None of the above

All of the above

I selected A and was incorrect, I am now lost on how to go about this. Thank You!

Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation exchange
chromatography. You add your protein to the column in a solution at pH of 3 and collect the eluant as fraction
A. You then wash the column with a buffer at pH 5 and collect the eluant as fraction B. You do a third wash of
your column with buffer at pH of 7 and collect the eluant as fraction C. You do a fourth and final wash of the
column with buffer at pH 12 and collect the eluant as fraction D. Which fraction contains most of your
protein?
O Fraction A
O Fraction B
O Fraction C
O Fraction D
O None of the above
O All of the above

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