1. You are extracting DNA from 10 mL of whole blood. WBC's (white blood cells) are collected via centrifugation. WBC concentration is 8 x 106 cells / mL. Answer the following questions. a. How many WBC's can you collect from the 10 mL of whole blood?

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can you please help with question 1. Thank you!

1. You are extracting DNA from 10 mL of whole blood. WBC's (white blood cells) are collected via
centrifugation. WBC concentration is 8 x 106 cells / mL. Answer the following questions.
a. How many WBC's can you collect from the 10 mL of whole blood?
g
b. The human genome (haploid) size is 3 x 10º base pairs (bp). The molecular weight of DNA is 660
How much does one copy of human genome weigh? (HINT: How many molecules are
mol x bp
there in one mole of a substance?):
C.
How much does DNA of a WBC weigh? (Note that WBCs are diploid.)
d. How much DNA can you collect from 10 mL of whole blood? (Assume that there is no loss
during DNA extraction.)
e. The purification of DNA allows scientists to perform many powerful genetic techniques in the
fields of medicine and forensics. Describe at least two different techniques that can be performed
once DNA has been isolated.
Transcribed Image Text:1. You are extracting DNA from 10 mL of whole blood. WBC's (white blood cells) are collected via centrifugation. WBC concentration is 8 x 106 cells / mL. Answer the following questions. a. How many WBC's can you collect from the 10 mL of whole blood? g b. The human genome (haploid) size is 3 x 10º base pairs (bp). The molecular weight of DNA is 660 How much does one copy of human genome weigh? (HINT: How many molecules are mol x bp there in one mole of a substance?): C. How much does DNA of a WBC weigh? (Note that WBCs are diploid.) d. How much DNA can you collect from 10 mL of whole blood? (Assume that there is no loss during DNA extraction.) e. The purification of DNA allows scientists to perform many powerful genetic techniques in the fields of medicine and forensics. Describe at least two different techniques that can be performed once DNA has been isolated.
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Introduction:

DNA: The entity who is a carrier of genetic information is known as DNA. It is also known as a self-replicating material which is available in all living organisms in the chromosomes. They are also known as Deoxyribonucleic acid in a scientific language.

WBC: WBCs are the one of the important types of blood cells that are generally found in lymph tissues and blood. They develop in the bone marrow. WBCs mainly involved in the fighting against various infections and helps in curing disease. These cell plays important role in body's immune mechanism.

The term “WBC Concentration” is nothing but checking the concentration of cells present in the available blood sample.

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