At which pH value does the amino group of amino acid have the best buffering capacity? 9.00 9.69 1.53 6.01 3.00 10.5 E. What is the pI (isoelectric point)? pI=
At which pH value does the amino group of amino acid have the best buffering capacity? 9.00 9.69 1.53 6.01 3.00 10.5 E. What is the pI (isoelectric point)? pI=
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
This is all part of one question. It goes together. (I wrote D and E that were cut off from the picture.) Please help me solve this since it all follows a line.
D. At which pH value does the amino group of amino acid have the best buffering capacity?
9.00
9.69
1.53
6.01
3.00
10.5
E. What is the pI (isoelectric point)?
pI=

Transcribed Image Text:The graph represents the titration of an amino acid with NaOH
solution.
A. At what pH are about 1/2 of the carboxyl groups ionized?
pH =
6.015
Incorrect
B. Where does the amino acid have a net charge of -1?
12
11
10
9
8
7
6.
5
4
3
دیا
N
1
0
T
I
I
pl = 6.01
PK₁ = 2.34
0.5
1.0
pK₂ = 9.69
1.5
OH-(equivalents)
2.0

Transcribed Image Text:B. Where does the amino acid have a net charge of -1?
above pH 9.69
at pH = 9.69
at pH = 6.01
at pH = 2.34
below pH 2.34
C. At what point has enough base been added to react with 1/2 of the NH3 groups?
1/2 of NH3 reacted =
7.85
Incorrect
when 1/2 of NH has reacted, the pH =
7.85
Incorrect
D. At which pH value does the amino group of amino acid have the best buffering capacity?
equivalents OH
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Step 1: What information does the titration curve provide?
VIEWStep 2: A. pH at which half of the carboxyl groups are ionized
VIEWStep 3: B. pH range at which the amino acid has -1 net charge
VIEWStep 4: C. point where half of amino group have reacted with the base
VIEWStep 5: D. pH at which the amino group have best buffering action
VIEWStep 6: E. Calculating pI
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