Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. Th total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18. 10° positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pks of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to ge the isolated product and regenerate the ionic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022 10. Don't look for formulas for this problem- we haven't gone over any

Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. Th total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18. 10° positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pks of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to ge the isolated product and regenerate the ionic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022 10. Don't look for formulas for this problem- we haven't gone over any

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to get the isolated product and regenerate the lonic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022* 10". Don't look for formulas for this problem- we haven't gone over any formulas for this. You can work through this problem by understanding how ion exchange works and using logic and dimensional analysis; the units on variables will be helpful in getting to the answer.
Transcribed Image Text:Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4 * 10° positively charged amine groups that can adsorba negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. 2. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to get the isolated product and regenerate the lonic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022* 10". Don't look for formulas for this problem- we haven't gone over any formulas for this. You can work through this problem by understanding how ion exchange works and using logic and dimensional analysis; the units on variables will be helpful in getting to the answer.
Expert Solution
steps

Step by step

Solved in 4 steps with 24 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS