A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent B-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between subunits. A B - BME + BME STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 8 Trypsin inhibitor, 20,000 10 cm migrated 2.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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A protein gives, under conditions of buffer composition, pH, and tem-
perature that are close to physiological conditions, a molecular weight by
size exclusion measurements of 140,000 g/mol. When the same protein
Transcribed Image Text:A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein
is studied by SDS gel electrophoresis in the absence or presence of the
reducing agent B-mercaptoethanol (BME), the patterns seen, respectively,
in lanes A and B are observed. Lane C contains standards of molecular
weight indicated. From these data, describe the native protein, in terms of
the kinds of subunits present, the stoichiometry of subunits, and the kinds
of bonding (covalent, noncovalent) existing between subunits.
A
B
- BME
+ BME
STD
Serum albumin,
67,000
Ovalbumin,
43,000
Carbonic anhydrase,
30,000
8
Trypsin inhibitor,
20,000
10
cm migrated
2.
Transcribed Image Text:is studied by SDS gel electrophoresis in the absence or presence of the reducing agent B-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. From these data, describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between subunits. A B - BME + BME STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 8 Trypsin inhibitor, 20,000 10 cm migrated 2.
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