Final Protein Concentration: 8 uM Final Gdn HCI Concentration (8 M stock) uL Gdn HCI stock uL HAstV-1 stock uL Storage Buffer Final Volume (uL) 50 0.25 50 0.5 50 1 50 1.5 50 2 2.5 25 50 50 3 3.5 4 4.5 5.25 5.5 5.75 6 50 50 50 50 50 50 50 50 50
stock aliquot - 1mg/ml
use the sequence below to determine the molarity using ExPASy Translate and protProt Program
atgggcgaggagtataaagttgtcctcacatttggatccccaatgagccctaatgcaaataacaaacagacttgggttaataaacctcttgatgcgccttcgggccattacaatgtgaaaattgcaaaggatgttgaccactatctaaccatgcagggtttcacttctatagcatctgttgactggtacactatagattttcaaccatctgaggcgcctgccccgataaaaggcttgcaggtacttgtgaacatctcgaaaaaagctgatgtgtatgccgtcaaacaatttgtcacagcgcagaccaacaacaagcaccaggttacaagcctgttcctagtaaaagtaacaactggttttcaggtgaacaactacctgagttacttttacagggcgtctgctactggggatgccacaactaacctgttggttagaggagacacatacacagcagggataagttttacccagggtggatggtatttgttgacaaatacatctattgttgatggggctatgccacctggctgggtctggaataacgtggaacttaaaactaacacagcgtatcacatggacaaaggcttggtccatctaataatgcctttgcctgagtccacgcaaatgtgttatgagatgctgacatctattccagcggccgcagagctcgctctggtgccacgcggtagttccgctcatcaccaccatcatcaccatcaccaccact
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