Calculate the specific activity and kcat for this enzyme

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Specific activity= Mole of Product
Min X Enzyme (mg)
Kcat= Mole of Product
Min X Mole of Enzyme
Example: - You used 0.5mg of enzyme total
- molecular mass of your enzyme is 80KDA (Why should you care?)
- Vmax=1.6mM/min
- volume of
your assay = 1ml
Calculate the specific activity and kcat for this enzyme
Transcribed Image Text:Specific activity= Mole of Product Min X Enzyme (mg) Kcat= Mole of Product Min X Mole of Enzyme Example: - You used 0.5mg of enzyme total - molecular mass of your enzyme is 80KDA (Why should you care?) - Vmax=1.6mM/min - volume of your assay = 1ml Calculate the specific activity and kcat for this enzyme
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