A mixture of four a-[3?P]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication, and incorporation into high-molecular-weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation a 1000-fold excess of unlabeled ribonu- cleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled INTPS added? (b) How could you tell that radioactivity is being incorporated as ribonucleotides, rather than an alternative such as reduction to deoxyribonucleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm

A mixture of four a-[3?P]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication, and incorporation into high-molecular-weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation a 1000-fold excess of unlabeled ribonu- cleoside triphosphates was added, with the results shown in the graph. Excess rNTPs added 5 10 15 Time, minutes (a) Why was the excess of unlabeled INTPS added? (b) How could you tell that radioactivity is being incorporated as ribonucleotides, rather than an alternative such as reduction to deoxyribonucleotides, followed by incorporation? (c) What does this experiment tell you about the process of DNA replication? Incorporation of radioac tivity, cpm

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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