Table 1. Purification of rat liver histidase. Purification step Homogenate Heating at 65 °C Ammonium sulfate fractionation (1) DEAE-Sephadex column Ammonium sulfate fractionation (II) QAE-Sephadex column Protein mg 17,718.1 3,097.7 503.8 71.9 26.9 0.232 Total activity nmol/min 12,138.0 8,751.1 8.335.8 2,156.7 1,730.9 79.5 Specific activity nmol/min per mg 0.685 2.8 16.5 30.0 64.3 342.7 Purification -fold 1 4 24 44 94 500.3 Yield % 100 72 68 18 14 0.65 Rev Invest Clin 2004; Vol. 56(1):43-50; Producción de histidasa recombinante de hígado de rata; Victor Ortiz, Nimbre Torres, Armando R. Tovar. A) In the table above, what columns provide evidence that the purity of the protein is increasing with each protein purification step? B) There is always a trade-off. As the purity of a protein INCREASES, what factors DECREASE? C) Based on your answer to part B, above, what is the ultimate limiting factor to the level of purity of a protein?
Table 1. Purification of rat liver histidase. Purification step Homogenate Heating at 65 °C Ammonium sulfate fractionation (1) DEAE-Sephadex column Ammonium sulfate fractionation (II) QAE-Sephadex column Protein mg 17,718.1 3,097.7 503.8 71.9 26.9 0.232 Total activity nmol/min 12,138.0 8,751.1 8.335.8 2,156.7 1,730.9 79.5 Specific activity nmol/min per mg 0.685 2.8 16.5 30.0 64.3 342.7 Purification -fold 1 4 24 44 94 500.3 Yield % 100 72 68 18 14 0.65 Rev Invest Clin 2004; Vol. 56(1):43-50; Producción de histidasa recombinante de hígado de rata; Victor Ortiz, Nimbre Torres, Armando R. Tovar. A) In the table above, what columns provide evidence that the purity of the protein is increasing with each protein purification step? B) There is always a trade-off. As the purity of a protein INCREASES, what factors DECREASE? C) Based on your answer to part B, above, what is the ultimate limiting factor to the level of purity of a protein?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:**Table 1. Purification of rat liver histidase.**
| Purification step | Protein mg | Total activity nmol/min | Specific activity nmol/min per mg | Purification -fold | Yield % |
|-------------------------------------------|------------|-------------------------|-----------------------------------|--------------------|---------|
| Homogenate | 17,718.1 | 12,138.0 | 0.685 | 1 | 100 |
| Heating at 65 °C | 3,097.7 | 8,751.1 | 2.8 | 4 | 72 |
| Ammonium sulfate fractionation (I) | 503.8 | 8,335.8 | 16.5 | 24 | 68 |
| DEAE-Sephadex column | 71.9 | 2,156.7 | 30.0 | 44 | 18 |
| Ammonium sulfate fractionation (II) | 26.9 | 1,730.9 | 64.3 | 94 | 14 |
| QAE-Sephadex column | 0.232 | 79.5 | 342.7 | 500.3 | 0.65 |
*Reference:* Rev Invest Clin 2004; Vol. 56(1):43-50.
*Producción de histidasa recombinante de hígado de rata.*
*Authors: Victor Ortiz, Nombre Torres, Armando R. Tovar.*
**A) In the table above, what columns provide evidence that the purity of the protein is increasing with each protein purification step?**
**B) There is always a trade-off. As the purity of a protein INCREASES, what factors DECREASE?**
**C) Based on your answer to part B, above, what is the ultimate limiting factor to the level of purity of a protein?**
### Explanation
The table presents data on the purification process of rat liver histidase, listing various steps involved, along with measurements for protein mass, total activity, specific activity, purification fold, and yield percentage. The increase in specific activity and purification fold numbers, alongside a decline in total protein and yield, indicates increased purity through consecutive
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