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Titration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.

Titration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Titration curve of an unknown amino acid

The graph below shows a curve, which was obtained following titration of an unknown amino acid.

  • Include a suitable descriptive title stating the identity of the unknown amino acid
  • Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions;
  • indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.
Excel File Edit View Insert Home Paste Chart 3 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 AutoSave OFF Insert Draw Page Layout A Calibri (Body) B I U v X ✓ fx Sheet1 + B Ready Accessibility: Investigate V v 16 Format Tools Data Window Help 20 Formulas Data Review View Automate V V Α΄ Α΄ Av C Hd E 14.0 13.0 12.0 11.0 10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0 D 0.0 0.5 General E Chart Design Format V 1.0 % Book1 A ←.0 .00 .00 →.0 F 1.5 OH equivalents 2.0 Tell me Conditional Format Formatting as Table W 2.5 G V X Cell Styles 3.0 Insert v Delete v Format v H * → ↓ V 97% AP Sort & Filter I o V Find & Select 3 Sun 16 Apr 9:47 am Comments Analyse Sensitivity Data J %D Share + 100% K
Transcribed Image Text:Excel File Edit View Insert Home Paste Chart 3 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 AutoSave OFF Insert Draw Page Layout A Calibri (Body) B I U v X ✓ fx Sheet1 + B Ready Accessibility: Investigate V v 16 Format Tools Data Window Help 20 Formulas Data Review View Automate V V Α΄ Α΄ Av C Hd E 14.0 13.0 12.0 11.0 10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0 D 0.0 0.5 General E Chart Design Format V 1.0 % Book1 A ←.0 .00 .00 →.0 F 1.5 OH equivalents 2.0 Tell me Conditional Format Formatting as Table W 2.5 G V X Cell Styles 3.0 Insert v Delete v Format v H * → ↓ V 97% AP Sort & Filter I o V Find & Select 3 Sun 16 Apr 9:47 am Comments Analyse Sensitivity Data J %D Share + 100% K
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