Calculate protein concentration in unknown samples 1, 2, 3:Absorbance of Unknown 1 = 0.541Absorbance of Unknown 2 = 0.85Absorbance of Unknown 3 = 1.02Standard Curve: Y = 0.0073x The table displayed is used to record data from an experiment involving bovine serum albumin (BSA) at a concentration of 1.0 mg/ml. The variables in the table are as follows:1. **BSA Volume (µl):** Quantities of BSA solution used, ranging from 0.0 to 50.0 µl.2. **[BSA] (µg/ml):** Calculated concentration of BSA, with values from 10 to 50 µg/ml.3. **Absorbance at 595 nm:** Measured absorbance values, showing how much light is absorbed at a wavelength of 595 nm. The values range from 0.371 to 1.253.4. **Net Absorbance at 595 nm:** Calculated by subtracting the blank absorbance from the sample absorbance. The data shows values between 0.188 and 0.882.Below the table, a calculation is shown for converting BSA volume into concentration:- Formula: \( x = \frac{1 \text{mg} \times 10 \mu\text{l}}{1 \text{ml}} \)- Result: \( x = 10 \mu\text{g}/\mu\text{l} \)There is also a note indicating that the "Net Absorbance" is calculated as "sample - blank." An example calculation given is:- \( (0.707 - 0.371) \), leading to a net absorbance of 0.336.This data is commonly used to create calibration curves for estimating the concentration of proteins in samples using spectrophotometric analysis.

Given that the standard BSA concentration is 1mg/mL. 10, 20, 30 ,40 and 50 µL of the standard BSA…

Answered: Calculate protein concentration in…

Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073x

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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