You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel filtration): 217 kDa Standard curve equation based on your protein ladder: 5205.6x^(-1.22) Number of bands in your unknown protein sample: 3 Distance each band traveled on your gel: A measured at 20 mm B measured at 62 mm C measured at 82 mm What is the molecular weight of subunit C? 24 kDa 29 kDa 34 kDa 43 kDa 135 kDa 145 kDa

Q: Based on this table:  1) What is the difference between subunit mass and native mass?  2) How do…

A: The molecular weight and the isoelectric points are characteristics of the protein that help in the…

Q: Distinguish the two methodologies used for getting a complete protein structure. Will the structural…

A: Proteins are essential for every cellular process occurring in a biological system as they control…

Q: You perform a size exclusion chromatography experiment and generate the following standard curve.…

A: In the question Kav vs molecular weight plot is given and the resulting linear equation is: y =…

Q: What is a Ramachandran Plot?

A: Ramachandran plot is developed by G N Ramachandran, C Ramakrishnan and V Sasisekharan in 1963. This…

Q: On an SDS-gel, If the distance traveled by the bromophenol blue dye is 7 cm, and the distance…

A: Given values:  Distance traveled by the dye front = 7 cm Distance traveled by the protein = 2.8 cm

Q: Physical methods are often used to determine protein conformation. Describe how x-ray…

A: Proteins: It is the most abundant organic molecule in living systems. It plays a role in structural,…

Q: Some characteristics of three proteins are listed in the table below: Protein Molecular Weight…

A: Chromatography is a separation technique that separates a mixture of analytes based on their…

Q: In order to prepare a standard curve, you will do serial dilutions of the standard protein. Assume…

A: Serial dilutionsIt refers to the stepwise dilution of a solution to create a series of solutions…

Q: Dra

A: Introduction: The genetic material that contains the instructions for the growth and operation of…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: If you crate the absorbance spectra of a solution containing dna protein and buffer using buffer as…

A: The absorbance spectrum is formed by plotting the Absorption values against the wavelength at which…

Q: How many copies of a protein need to be present in a cell in order for it to be visible as a band on…

A: * Given that you can load 100 µg of cell extract onto a gel and can detect 10 ng in a single band by…

Q: Describe and explain sources of errors in this experiment. Provide practical suggestions for…

A: Protein solubility refers to the ability of a protein to dissolve in a solvent such as water, and is…

Q: You are purifying a protein for the first me and decide to analyze it on SDS PAGE. You run two lanes…

A: A. This finding implies that the protein likely has disulfide links in its natural state. Disulfide…

Q: You want to make 94 µL of the diluted Cell-Free extract for estimation of total protein. To do this,…

A: A ratio of 1:25 means add 1 part of product to 25 parts of water. Dilution factor does not gives…

Q: This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses…

A: SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Q: A C2 G This a photograph of an actual polyacrylamide gel that has been run with samples of protein.…

A: Note: Hi! Thank you for the question. We are authorized to answer three subparts at a time. Since…

Q: Several calibration curves were created for a series of protein standards of known molecular mass…

A: Introduction :- Chromatography is a laboratory method used in chemical analysis to separate a…

Q: A 0.5µM solution of the protein in 75mL is what you want. In micrograms, how much of the protein do…

A: Molar concentration is a measure of the concentration of a solute in solution with respect to the…

Q: In isoelectric focusing, a protein sample is applied at the end of a gel with an immobilized pH…

A: In isoelectric focusing, a protein sample is applied at the end of a gel with an immobilized pH…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541…

A: Given that the standard BSA concentration is 1mg/mL. 10, 20, 30 ,40 and 50 µL of the standard BSA…

Q: A protein is purified from a bacterium using Size Exclusion Chromatography (SEC), with a molecular…

A: It is a sieve tube chromatography technique, in which proteins are separated from other proteins…

Q: A protein has a molecular weight of 24kDa.  You want a 0.5µM solution of the protein in 75mL.  How…

A: Given information Protein molecular weight- 24kDa The concentration of the solution to be made-…

Q: Given that the protein concentration is 0.25 μM, what is the association constant (KA) of the…

A: Fluorescence is a luminescence technique used in fluorescent labeling and fluorescence spectroscopy…

Q: Physical methods are often used to determine protein conformation. Describe how x-ray…

A: Proteins- amino acids are the monomers for the production of proteins and contain nitrogen with…

Q: (a) 1 Normalized fluorescence 0.8 0.6 0.4 0.2 0 50 55 OM 0.100 M 0.200 M 0.300 M 0.500 M 1.00 M 2.00…

A: There are four classes of biological macromolecules - proteins, nucleic acid, lipids and…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: Does this calculation look correct? My goal isto have a target mass of 10 µg of my protein with a…

A: Yes this calculations look correct.since you want your final volume to be 30 microlitres you have to…

Q: You are given a pure protein sample to characterize and provided the following information: Its…

A: Introduction  One or more long chains of amino acid residues make up the massive biomolecules and…

Q: You have a soluble protein that is highly flexible and is only 23 kDa in size. What is the most…

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Q: You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and…

A: Polyacrylamide gel electrophoresis is a technique in which proteins are migrated and separate out…

Q: 1.0 0.9 Z 0.8 15. A pharmaceutical company studied the binding of three different compounds, X, Y,…

A: Compound Z has the strongest affinity for the protein. This is indicated by the higher fractional…

Q: Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin…

A: Electrophoresis is a process of separating the biological molecules such as DNA, RNA and protein on…

Q: The following data is protein melting point (Tm) characterization data obtained by the Zetasizer…

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Q: Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. A…

A: On basis of the gel used, electrophoresis is classified into 2 types, Agarose gel…

Q: purified Protein X' and you want to know its concentration. As we learned, you can calculate…

A: Absorbance = 0.6 Absorptivity = 0.2 ml/mg cm Path length = 1cm

Q: Compute for the albumin to globulin (A/G) ratio using these data given Albumin= 51.9g/L Total…

A: Albumin  Globulin ratio is the ratio of albumin to globulin in the serum. The Albumin to Globulin…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.