You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and Porcine pepsin (pl: 1.0) in a Tris-Glycine buffer (pH 5.2). (pl = Isoelectric point). Select which of the following is true. O All proteins will migrate at the same rate O All proteins will migrate at the same direction Porcine pepsin will migrate toward the electrode Porcine pepsin will not migrate and stay in the well O O O O
You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and Porcine pepsin (pl: 1.0) in a Tris-Glycine buffer (pH 5.2). (pl = Isoelectric point). Select which of the following is true. O All proteins will migrate at the same rate O All proteins will migrate at the same direction Porcine pepsin will migrate toward the electrode Porcine pepsin will not migrate and stay in the well O O O O
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Question
Transcribed Image Text:**Electrophoresis of Proteins Experiment Analysis**
In this experiment, we explore the electrophoresis of three proteins: Hemoglobin, DNA Polymerase, and Porcine Pepsin. The isoelectric points (pI), where each protein has no net charge, are as follows: Hemoglobin (pI: 6.8), DNA Polymerase (pI: 5.2), and Porcine Pepsin (pI: 1.0). The medium utilized for this experiment is a Tris-Glycine buffer with a pH value of 5.2.
**Electrophoresis Conditions and Expected Outcomes:**
Electrophoresis is a technique used to separate proteins based on their size and charge. In this context, since the buffer pH is 5.2, the behavior of each protein can be predicted as follows:
1. **Hemoglobin (pI: 6.8)**: At pH 5.2, which is lower than its pI, Hemoglobin will have a net positive charge. Therefore, it will migrate towards the negative electrode (cathode).
2. **DNA Polymerase (pI: 5.2)**: Its pI is equal to the buffer pH. In such a scenario, DNA Polymerase will have no net charge and, thus, will not migrate significantly in the electric field.
3. **Porcine Pepsin (pI: 1.0)**: Since pH 5.2 is higher than its pI, Porcine Pepsin will have a net negative charge, causing it to migrate towards the positive electrode (anode).
### Question Presented:
Select which of the following statements is true based on the described conditions:
- All proteins will migrate at the same rate
- All proteins will migrate in the same direction
- Porcine Pepsin will migrate towards the electrode
- Porcine Pepsin will not migrate and stay in the well
**Answer Analysis:**
Given the behavior of each protein at pH 5.2:
- **Statement 1**: Incorrect, as proteins migrate at different rates based on their charges.
- **Statement 2**: Incorrect, as the direction of migration depends on the charge of the protein.
- **Statement 3**: Correct, Porcine Pepsin will indeed migrate towards the positive electrode.
- **Statement 4**: Incorrect, Porcine
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