In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein. The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration.Protein (ug)Absorbance at 595nm0.000.4669.360.67618.720.88328.081.08637.441.280 Use Excel to determine the equation of the line put it here in the format of y = mx + b. Start your answer with "y=".Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973. Report answer to the hundredths place.

Step 1:Beer's law equation is A=ϵClA is absorbance, C is concentration.Now Absorbance is the…

Answered: In the Bradford protein determination,…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein. The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration.

Protein (ug)	Absorbance at 595nm
0.00	0.466
9.36	0.676
18.72	0.883
28.08	1.086
37.44	1.280

Use Excel to determine the equation of the line put it here in the format of y = mx + b. Start your answer with "y=".

Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973.

Report answer to the hundredths place.

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