In the Bradford protein determination, the color of a dye changes from brown to blue when it binds to a protein.  The intensity of blue color (measured by absorbance of light at a wavelength of 595nm) is proportional to protein concentration. Protein (ug) Absorbance at 595nm 0.00 0.466 9.36 0.676 18.72 0.883 28.08 1.086 37.44 1.280   Use Excel to determine the equation of the line put it here in the format of y = mx + b.  Start your answer with "y=". Using the calibration curve for the Bradford protein determination in the previous question, determine the number of micrograms of protein in an unknown if it gave an absorbance of 0.973.  Report answer to the hundredths place.

Q: Suppose you calculate that you can load 2000ng of Sample A and suppose your concentration of Sample…

A: * We must figure out how much Sample B is needed to give 2000ng of protein in order to compute the…

Q: Which of the following must be true based on the data below for ligand L binding to protein A and to…

A: Proteins are biomolecules which catalyzes most of the biochemical reactions in all living cells and…

Q: If the target protein is 0.1% of the total protein in the original mixture, a three-step…

A: Purification is a process by which impurities are removed from a sample and desired component is…

Q: In order to prepare a standard curve, you will do serial dilutions of the standard protein. Assume…

A: Serial dilutionsIt refers to the stepwise dilution of a solution to create a series of solutions…

Q: The figure shows binding curves for two proteins that bind the same ligand. Which binding curve…

A: Ligand binding curve: this is also called as saturation curve that shows the fraction Y of protein…

Q: Which of the following is correct concerning the oxygenation plot of proteins X and Y shown below?

A: There are a few important points.Oxygen will not dissolve easily in water. About 1.5% of inhaled…

Q: Denatured protein is in a low energy state. What sort of explanation can you use to rationalize that…

A: Denaturation of protein: When proteins are denaturized, they lose the quaternary, tertiary, and…

Q: A solution contains a mixture of four proteins: urease, ovalbumin, lysozyme, and alkaline…

A: Proteins are composed of a linear chain of amino acids attached together via peptide bonds. There…

Q: You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel…

A: To determine the molecular weight of subunit C from the SDS-PAGE data, we'll use the provided…

Q: 16. A quantitative study of the interaction of a protein with its ligand yielded the following…

A: I cannot plot the graph as that would be doing your homework.Determine KD:From the graph, read the…

Q: Based on the Hill Plot below, this protein-ligand interaction displays log Y 1-Y 2.5 0 -2.5 -2.5…

A: Hill equation developed by Archibald Hill in 1910 quantitatively describing cooperative ligand…

Q: You want to treat 10 mL of HL-60 cells with cycloheximide in order to determine the half-life of a…

A: According to Normality Equation,

Q: Suppose you have a mixture of the following proteins protein A: pl = 3.5, mw = 35 kDa protein B: pl…

A: Cation exchange chromatography is defined as part of the ion-exchange chromatography. It is used to…

Q: Using the chart below, can you produce a two-step procedure that demonstrates protein purification…

A: Proteins are polymers of amino acids linked by peptide bonds. Charge on the protein depends on the…

Q: Given that the protein concentration is 0.25 μM, what is the association constant (KA) of the…

A: Fluorescence is a luminescence technique used in fluorescent labeling and fluorescence spectroscopy…

Q: Which way is more common practice to characterize the strength of a binding reaction between a…

A: Answer : the more common practice to characterize the strength of a binding reaction between a…

Q: Calculate the concentration of the ligand required to fill 25% of the binding site.

A: Given, X+A ⇌ XA       , ka = 1.5 ×10-6 Y+A ⇌ YA         , ka = 4.7 × 10-4 Kd = 1Ka So,  Kd for first…

Q: Suppose that you are tasked to determine the protein concentration of an unknown protein solution…

A: The concentration of unknown protein solution can be determined by plotting a graph between…

Q: (a) 1 Normalized fluorescence 0.8 0.6 0.4 0.2 0 50 55 OM 0.100 M 0.200 M 0.300 M 0.500 M 1.00 M 2.00…

A: There are four classes of biological macromolecules - proteins, nucleic acid, lipids and…

Q: A spheroidal bacterium with a diameter of 1.0 mm (micrometer, 1 mm = 10-6) contains 25,000 molecules…

A: A spheroidal bacterium are circular or oval in shape which are generally known as coccus (plural…

Q: The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein…

A: Proteins are amazing molecules that make everything simple inside the cell. Protein molecules are…

Q: Two proteins bind the same ligand, and the data is shown below. What is the KD value for Protein B?…

A: The answer of the above question is: Correct answer not given. KD is the equilibrium dissociation…

Q: Imagine that you were asked to denature a protein; you know you can do so using urea.  Your protein…

A: Denaturation is the process by which a protein looses it native structure, to the level that protein…

Q: The pH vs charge graph for a triprotic amino acid is shown below. Please answer the following…

A: An amino acid with the ability to donate 3 protons (3 H+) is called a triprotic amino acid. The 3…

Q: Make a table with a scale of absorbance and the concentration of protein in Chromatin sample from…

A: Since you have posted multiple questions, we will provide the solution only to the first question as…

Q: You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and…

A: Polyacrylamide gel electrophoresis is a technique in which proteins are migrated and separate out…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.