a) The pl of a protein is 9.0. What will be thenet charge (positive, negative or neutral) onthe protein under the following conditions:A buffer with pH 9.0:A buffer with pH 7.0:A buffer with pH 10.0:b) Your protein is present in a sample buffer which has a pH 7.0. The sample is applied to an ANIONEXCHANGE column. Will protein 1 bind to the beads on the column?Yes/No

Answered: a) The pl of a protein is 9.0. What…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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a) The pl of a protein is 9.0. What will be the
net charge (positive, negative or neutral) on
the protein under the following conditions:
A buffer with pH 9.0:
A buffer with pH 7.0:
A buffer with pH 10.0:
b) Your protein is present in a sample buffer which has a pH 7.0. The sample is applied to an ANION
EXCHANGE column. Will protein 1 bind to the beads on the column?
Yes/No

Expert Solution

Step 1

Proteins are macromolecules that are formed by several amino acids. Amino acids carry different charges on the side chains. Hence, they can be positive, negative or neutral. Thus, the net protein charge is based on the charges of the amino acids.

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