Using mass spec, the primary structure of 4 peptides is obtained from a tissue sample: VMDEL, GHKRT, CNPQI, and SAWFY. The pI of each peptide is calculated and ion exchange chromatography is used to purify each peptide. VMDEL, GHKRT, CNPQI, and SAWFY were separated into 4 pure samples. The labels on all the samples are erased by mistake, and the mass spec is no longer working. You can run a few simple tests to identify the samples. What are 4 tests to identify each peptide and why?
Using mass spec, the primary structure of 4 peptides is obtained from a tissue sample: VMDEL, GHKRT, CNPQI, and SAWFY. The pI of each peptide is calculated and ion exchange chromatography is used to purify each peptide. VMDEL, GHKRT, CNPQI, and SAWFY were separated into 4 pure samples. The labels on all the samples are erased by mistake, and the mass spec is no longer working. You can run a few simple tests to identify the samples. What are 4 tests to identify each peptide and why?
Amino acid is the basic unit of a protein. An amino acid contains carboxylic acid groups (—COOH), amino groups (—NH2), and substituent or replaceable side chains (—R). There are 20 different amino acids present in the body.
Amino acids can be classified as neutral, basic, or acidic in accordance with their side chains. The bond between two amino acids is an amide bond, and in a protein this bond is called a peptide bond.
Step by step
Solved in 3 steps
