4 You are analyzing the peptide ala-ile-glu-lys-phe-val-tyr-cys. If you treat the peptide with chymotrypsin,which technique would be best to separate thefragments?O a. ultracentrifugationO b. affinity chromatographyO c. dialysisd. gel filtration chromatographye. ion exchange chromatographyWhat is an advantage of NMR spectroscopy over x-raycrystallography in studying protein structure?

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Answered: You are analyzing the peptide…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.1: Introduction To Clinical Chemistry

Problem 9RQ

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Pathogenic E. coil have recently been shown to degrade tight junction proteins during infection. How would this provide an advantage to the bacteria?
b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Denote the point in the pathway in which each individual is blocked.
a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Draw the pathway leading to the production of protein E.
In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.
How long would the peptide be that is translated from this MILNA sequence: 5-AUGGGCUACCGA-3? a. 0 b. 2 c. 3 d. 4
In the experiments of Aery, MacLeod, and McCarty, what was the purpose of treating the transforming extract with enzymes?
The synthetic process by which monomers are covalently linked is (a) hydrolysis (b) isomerization (c) condensation (d) glycosidic linkage (e) ester linkage
What are Okazaki fragments and how they are formed?

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