Top of gelStd MW's105,00084,00050,50037,00029,000DO21,000 This is a picture of an SDS-PAGE gel. In the left lane are protein standards withmolecular masses written on the left. The gel was cut off at the bottom at the dyefront. What is the Rf of the protein of the band enclosed with the dotted box? Whatis the approximate molecular mass of this protein?Top of gel

SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Answered: This is a picture of an SDS-PAGE gel.…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

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With thousands of different spots on the gel, how are the identities of the proteins ascertained?
an protein has a molecular wieght of 90 KD when purified by gel filtration and has a molecular wieght of 60 KD hen purified by electrophoresis which one is the more selective?
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.
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First, they ran samples of the enzyme on denaturing and non-denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where molecular weight standards of various sizes would occur if they’d been on the gels. How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?
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This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses written on the left. The gel was cut off at the bottom at the dye front. What is the Rf of the protein of the band enclosed with the dotted box? What is the approximate molecular mass of this protein?