For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C
For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you
calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for
Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no
decimal places). Units (°C) are already shown so must not be included in your answer.
Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3'
°C
Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3'
°C](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F23cfaf13-27cc-434f-9074-42f27a2b7b95%2F48d06d0f-ef20-4e59-8484-4a91c25522e1%2Fsi9od9_processed.png&w=3840&q=75)
Transcribed Image Text:For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you
calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for
Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no
decimal places). Units (°C) are already shown so must not be included in your answer.
Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3'
°C
Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3'
°C
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