For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question
100%
For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you
calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for
Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no
decimal places). Units (°C) are already shown so must not be included in your answer.
Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3'
°C
Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3'
°C
Transcribed Image Text:For another project, you designed primers 1 and 2 with the sequences shown below. Your lab partner suggests you calculate the primer melting temperatures (Tm) using the formula: Tm = [(A+T) x 2°C] + [(G+C) x 4°C]. Calculate the Tm for Primers 1 and 2 and give your answers in the boxes provided below. You must give your answer as a whole number (no decimal places). Units (°C) are already shown so must not be included in your answer. Primer 1 (forward): 5' TTCGTCTGGTGAACTCAG 3' °C Primer 2 (reverse): 5' CAGGGTATGTCCGTATAT 3' °C
Expert Solution
steps

Step by step

Solved in 2 steps with 2 images

Blurred answer
Knowledge Booster
Genomics
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education