Visually compare the intensity of your team’s Plant-DNA sample with the intensity of the 3 kb band on the DNA ladder (see figure posted below). For example, if your Plant- DNA sample closely matches the 3 Kb band, this represents approximately 125 ng of total DNA. Divide the approximate mass (ng) of DNA in your sample by the volume loaded in the gel minus the loading dye added (i.e., 12 – 2 = 10 μL, since you loaded 12 μL from a 24 μL sample that contained 20 μL DNA prep + 4 μL loading dye. What is the DNA concentration of your sample? ______ng/μL
Visually compare the intensity of your team’s Plant-DNA sample with the intensity of the 3 kb band on the DNA ladder (see figure posted below). For example, if your Plant- DNA sample closely matches the 3 Kb band, this represents approximately 125 ng of total DNA. Divide the approximate mass (ng) of DNA in your sample by the volume loaded in the gel minus the loading dye added (i.e., 12 – 2 = 10 μL, since you loaded 12 μL from a 24 μL sample that contained 20 μL DNA prep + 4 μL loading dye. What is the DNA concentration of your sample? ______ng/μL
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Visually compare the intensity of your team’s Plant-DNA
sample with the intensity of the 3 kb band on the DNA ladder
(see figure posted below). For example, if your Plant-
DNA sample closely matches the 3 Kb band, this represents
approximately 125 ng of total DNA.
Divide the approximate mass (ng) of DNA in your sample by
the volume loaded in the gel minus the loading dye added
(i.e., 12 – 2 = 10 μL, since you loaded 12 μL from a 24 μL
sample that contained 20 μL DNA prep + 4 μL loading dye.
What is the DNA concentration of your sample? ______ng/μL
Transcribed Image Text:Mass
(ng)
Kb
40 10.0-
40
49
40
32
120
40
57
45
122
34
31
27
23
124
49
37
32
8.0
6.0
5.0
4.0
3.0
1\\\\"
2.0-
1.5-
-
1.2-
1.0
0.9
0.8-
0.7-
0.6-
0.5-
0.4-
0.3-
0.2-
61 0.1-
Transcribed Image Text:#LCCE
#ICCE
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