Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse Primer Taq DNA polymerase DNA template Final Concentration (D) X 2mM (G) UM 0.5 mM 0.5 mM Volume (UL) Per 25ul Reaction (B) 2.5 1.0 0.5 0.25 (J) 0.2 2.0 Volume (ul) for (A)X reactions (C) 22.5 (F) (H) 2.25 2.25 (K) --

Reagents Sterile ddH₂0 10X PCR Buffer (E) mM MgCl₂ 10mM dNTP (1) mM Forward Primer 50 mM Reverse Primer Taq DNA polymerase DNA template Final Concentration (D) X 2mM (G) UM 0.5 mM 0.5 mM Volume (UL) Per 25ul Reaction (B) 2.5 1.0 0.5 0.25 (J) 0.2 2.0 Volume (ul) for (A)X reactions (C) 22.5 (F) (H) 2.25 2.25 (K) --

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

What is the difference between cost per billable test and cost per reportable result. (Give examples referring from the laboratory environment)
How would you calculate the expected values from the observed in order to use the chi-squared test?
A patient is to receive Tazidine by intramuscular injection. A 10.0-mL vial contains 1 gram of dry powder that was reconstituted with 3 mL of sterile water. The reconstituted solution contains 340 milligrams Tazidine in 1.00 mL of volume. How much solution should be injected if a patient needs to receive a 0.30 gram dose? Enter the numerical part of the answer in the first box (using correct significant figures) and the correct unit in the second box.
A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)
Create a diagram of the steps in the Most Probable Number test. You may insert a computer-made diagram, or you may draw a diagram by hand
A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?
Would you expect the results from the MAC test to corroborate the results from the EMB test? Explain why or why not.
A mixture of species of A and B is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Species Retention time (min) Peak Width at Base (min) Non-retained 3.0 A 5.2 0.48 В 5.9 0.56 From the data, the calculated resolution Rs is smaller than 1.5 for A and B when the column length is 20.0 cm, which indicates that A and B are not separate completely. What is the new column length (unit: cm) necessary to separate the A and B species with a good resolution Rs = 1.5? Please keep your answer to one decimal place.
When running a gel, the lane loaded with a negative control sample should will always show a band that lines up with the experimental band, regardless if the sample is contaminated. not show a band, which indicates the sample is contaminated. show a band, which indicates that the sample is not contaminated. not show a band, which indicates that the sample is not contaminated.
Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMP
You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer. You observe 32 stage micrometer units line up with 15 ocular micrometer units. What is the value for each division of the ocular micrometer for this objective? (Round to the 1 decimal place)
Which is the best method for producing phase-separated GUVs (at least 20 um in size)?