When one uses Benedict’s reagent added to UNKNOWN samples taken and added (as a liquid) by a pipette (in our homes we call them eye-droppers) from each container into a labeled by number test tube. The seven labeled test tubes are then immersed into a beaker on a hot plate with warm water in it (called a water bath): Samples : ONE, TWO, THREE, FOUR, FIVE, SIX, SEVEN All seven UNKNOWNS having Benedicts reagent added to them then change from transparent clear after to the same color as the Benedict’s reagent, when the blue colored reagent is added to them. They are all (all seven unknowns) now blue in color. Then the seven test tubes are immersed in a warm water bath. What is seen by you is that NO color change occurs initially when they are all immersed in warm water. THEN, over a five to ten-minute window of time as you watch the unknown test tube containing samples in the warm water bath, the test tubes containing unknowns ONE and FOUR change in color, to your eyes, to an identified a positive color change of ORANGE/RED/white. Is there a presence of: MONOSACCHARIDES (reducing sugar varieties as in glucose and fructose), PROTEINS (long, or short chain polypeptides) and LIPIDS: oils fats and waxes

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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When one uses Benedict’s reagent added to UNKNOWN samples taken and added (as a liquid) by a pipette (in our homes we call them eye-droppers) from each container into a labeled by number test tube. The seven labeled test tubes are then immersed into a beaker on a hot plate with warm water in it (called a water bath): Samples : ONE, TWO, THREE, FOUR, FIVE, SIX, SEVEN All seven UNKNOWNS having Benedicts reagent added to them then change from transparent clear after to the same color as the Benedict’s reagent, when the blue colored reagent is added to them. They are all (all seven unknowns) now blue in color. Then the seven test tubes are immersed in a warm water bath. What is seen by you is that NO color change occurs initially when they are all immersed in warm water. THEN, over a five to ten-minute window of time as you watch the unknown test tube containing samples in the warm water bath, the test tubes containing unknowns ONE and FOUR change in color, to your eyes, to an identified a positive color change of ORANGE/RED/white. Is there a presence of: MONOSACCHARIDES (reducing sugar varieties as in glucose and fructose), PROTEINS (long, or short chain polypeptides) and LIPIDS: oils fats and waxes
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