A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution wassubjected to a Bradford assay. The increase in absorbance at 595 nm relative to theconcentration of protein was plotted as shown below.Save1.0-0.8-0.6-0.4-0.2-0.040.00.51.02.02.51.5BSA concentration (mg/mL)a) Using the graph, calculate the original concentration of protein present in a mixture ofhaemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at awavelength of 595 nm using the Bradford assay. Show all workings.Absorbance (O.D.)

The Bradford assay is based on the fact that the absorbance maximum for an acidic solution of…

Answered: A series of bovine serum albumin (BSA)…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.
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Determine the concentration of monocytogenes enumerated from the apple slice based on the information below. You must draw your serial dilution and show your mathematical calculation to receive full credit. The apple slice (12 g) was first mixed with 108 ml of sterile 0.1% peptone water. After, 1 ml was added to tube containing 9 ml of sterile 0.1% peptone water. Process was repeated with two additional tubes. 1 ml was pipetted from tubes 2 and 3 onto MOX media. Plates were incubated at 37 for 48 hours. The pictures below show the plates from tube 2 (left) and tube 3 (right).
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Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.197, taking into account the dilution factor 100 per and total volume of extract 140ml
1
Hey I am practicing for a molecular biology test and I am having trouble with is practice problem. I am not sure how to set it up to determine the amount of Acid Phosphatase. Can you help? Determine the amount of Acid Phosphatase in micrograms/ gram wheat germ from the following experimental values. The amount of acid phosphatase found in 198 ul of extract was 8 ug. The amount of extraction buffer used was 8 mL and the amount of wheat germ was 0.8 g.
You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples; your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How did you determine that? BSA (ug/ml) Absorbance @ 595 nm. 25 0.15 50 0.30 75 0.45 100 0.60 150 0.90 200 1.25
Which fractions measured from your gel filtration experiment had the most and least amount of protein detected? Which fraction likely contained the most myoglobin based off molecular weight and protein presence? Which fraction likely contained the most egg albumin based off molecular weight and protein presence? Which samples measured from your ion exchange experiment had the most protein detected? Calculate the approximate pI of your proteins using the ion exchange Table 2. Ion Exchange Samples Tested by Bradford Assay Concentration BSA (μg/mL) Concentration BSA (mg/mL) Volume of Sample added to Bradford Reagent (μL) Absorbance at 595 nm Standard 1 250 0.25 50 0.32 Standard 2 125 0.125 50 0.16 Standard 3 62.5 0.0625 50 0.08 Standard 4 31.25 0.03125 50 0.04 Standard 5 15.625 0.015625 50 0.02 Blank 0.00 0.00 0.00 Table 3. Gel Filtration Fractions Tested by Bradford Assay Fraction #:…
Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280 nm. The sample was diluted (200 μL of purified antibody in 800 μL buffer) prior to analysis using a spectrophotometer. Calculate the concentration of antibody in the purified fraction if the Abs=0.95 of the diluted antibody. The molar absorptivity is known to be 191,411.6 M-1cm-1 and the molecular weight is 150 kDa. The pathlength for the cuvette is 1 cm.
You run 5 standard proteins listed below on a size-exclusion (gel filtration) column with limit of 200,000Da. Please draw a chromatogram on a separate page with each peak and both axes labeled. Protein B-Amylase Alcohol dehydrogenase Bovine albumin Carbonic anhydrase Cytochome A280 MW 223,800 0.5 82,000 0.6 66,463 0.45 30,000 0.43 12,000 0.8

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