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Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.2 0.02 0.04 0.3 0.112 0.167 0.4 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 0.176 PRE DESALTING 3 POST DESALTING 0.157 3

Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.2 0.02 0.04 0.3 0.112 0.167 0.4 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 0.176 PRE DESALTING 3 POST DESALTING 0.157 3

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please help even if its just an explanation on how to do this. I am so confused. Thank you so much 

3. Using the protein concentrations that you calculated, determine the volume in ul of each sample that would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row of the table. SAMPLE Volume of Vol of 4x Volume of Final total volume (uL) sample for 25ug (uL) Tris for gel loading sample buffer CRUDE 8. 25 17 50 FLOW THRU 50 PRE DESALTING 50 POST 50 DESALTING
Transcribed Image Text:3. Using the protein concentrations that you calculated, determine the volume in ul of each sample that would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row of the table. SAMPLE Volume of Vol of 4x Volume of Final total volume (uL) sample for 25ug (uL) Tris for gel loading sample buffer CRUDE 8. 25 17 50 FLOW THRU 50 PRE DESALTING 50 POST 50 DESALTING
1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve. Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.02 0.2 0.04 0.3 0.112 0.4 0.167 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR Iprotein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 PRE DESALTING 0.176 3 POST DESALTING 0.157 3
Transcribed Image Text:1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve. Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.02 0.2 0.04 0.3 0.112 0.4 0.167 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR Iprotein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 PRE DESALTING 0.176 3 POST DESALTING 0.157 3
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