2.4. Preparation of a Calibration CurveStandard Bovine Serum Albumin (BSA) solutionStock solution: 2.00 mg/mLStandard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSADiluent: distilled water a. on the pictureb.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL ofdistilled water. Mix using a vortex mixer.c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of theremaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions.Concentration to beprepared, mg/mLVolume of 2.00 mg/mLstock solution needed, μL Volume of distilledwater needed, μL0.0001000.00 0.12562.5937.50.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the picture a. Calculate the volume of stock solution needed. Use C.V1=C2V2.Sample calculation:How to prepare 1.00 mL of 0.125 mg/mL BSA solution from a 2.00 mg/mL stock solution?Use CVi=C2V2where CVi is the concentration and volume of the stock solution,and CV2 is concentration and volume of the solution to be prepared.So,V1=(C¿V2)/C,V;=(0.125 mg/mL x 1.00 mL)/2.00 mg/mL = 0.0625 mL = 62.5 µL of 2.00 mg/mLBSAORATORYtry and AgriVolume of distilled water = 1000 µL - 62.5 µl = 937.5 µL

Standard solutions are those that have a solute dissolved in a solute and have a known concentration…

Answered: Preparation of a Calibration Curve…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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2.4. Preparation of a Calibration Curve
Standard Bovine Serum Albumin (BSA) solution
Stock solution: 2.00 mg/mL
Standard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSA
Diluent: distilled water

a. on the picture

b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL of
distilled water. Mix using a vortex mixer.
c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of the
remaining concentrations following the sample calculation.

Table 2.4. Volumes needed for the preparation of the BSA standard solutions.

Concentration to be prepared, mg/mL	Volume of 2.00 mg/mL stock solution needed, μL	Volume of distilled water needed, μL
0.00	0	1000.00
0.125	62.5	937.5
0.25
0.50
1.00
1.50
2.00