3. Imagine that you inoculated 5 tubes of BGLB for each of three dilutions, and found a combination of positives of 4-4-2, which is not found on the MPN Index table. Your dilution series was 1 ml, 0.1 ml, and 0.01 ml. Estimate the MPN/100 ml value using the calculation found on the previous page.
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![3. Imagine that you inoculated 5 tubes of BGLB for each of three dilutions, and found a
combination of positives of 4-4-2, which is not found on the MPN Index table. Your dilution
series was 1 ml, 0.1 ml, and 0.01 ml. Estimate the MPN/100 ml value using the calculation
found on the previous page.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbda50206-2115-4a86-8204-509ce5f08e3a%2F94713448-a97a-4c27-9b62-8c1e3adab58d%2Fit42pp_processed.jpeg&w=3840&q=75)
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- Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1ml | | ||| 9ml 9ml 9ml 9ml 9ml Chloroform-treated lysate 101 102 103 104 105 0.1 ml 0.1 ml_0.1 ml Empty sterile tubes ||| + 0.5 ml E. coli Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix Molten soft agar overlay O00000 Bottom agar Number of plaque-forming units (pfu) Plate A Dilution Plate B 103 104 105 254 265 132 11 110 23 average number of plaques x dilution factor volume plated pfu mlAt the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
- You want to infect a plate of 2x10^6 HeLa cells with poliovirus at an MOI of 10. Your viral stock is at concentration of 8x10^7 particles/ml. What volume of your viral stock do you need to apply to your cells? Show your workingA B Which of the bacterial colonies shown (A or B) was nonlethal when injected in mice? А ВDuring incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?
- 1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.Since higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?a) What are the important parameters for the eluent selection in Gel Permeation Chromotography (GPC)? Explain your answer in detail. What is an universal calibration curve and what is the advantage of using it in GPC analysis?
- Calculate the viral titer of an experiment performed yielding a pfu of 279 from plating 1.0 ml of the 10-5 dilution. Show all work and write the answer in scientific notation.6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?