A cell count was performed on a serous fluid specimen.  As the fluid was cloudy, a 1:5 dilution was prepared.  The counts were: 34 and 32; 5 squares on each side were counted.  What is the average cell count per L?  Side 1:  34 x 1 x (1/(5x0.1)) x 5 = 340 per µl Side 2:  32 x 1 x (1/(5x0.1)) x 5= 320 per µl

Human Anatomy & Physiology (11th Edition)
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A cell count was performed on a serous fluid specimen.  As the fluid was cloudy, a 1:5 dilution was prepared.  The counts were: 34 and 32; 5 squares on each side were counted.  What is the average cell count per L? 

Side 1:  34 x 1 x (1/(5x0.1)) x 5 = 340 per µl

Side 2:  32 x 1 x (1/(5x0.1)) x 5= 320 per µl

Expert Solution
Step 1: Introduction

The subject is a cell count from a sample of serous fluid that was previously described as hazy. A 1:5 dilution was created to guarantee accuracy in the cell count. Two sides—Side 1 and Side 2—conducted the counting, each determining the presence of cells by counting five squares. 


After the count, it was intended to calculate the average cell count per liter (cells/L) using the information gathered. This computation is an essential step in determining the cell concentration in the sample of serous fluid, which is important for many medical and scientific purposes.

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