Given the following values, use the absorbance formula to find the hemoglobin concentration of the unknown.As =0.600Au =0.300Cs =20g/dl 5. Place the tube in a comparator block and add distilled water dropby drop until it matches the Hb standard in the comparator block.6. Read the meniscus directly on the gram scale of the tube.GRAVIMETRIC Method:Copper Sulfate MethodPrincipleSpecific gravity of the blood is largely determined by the amount ofhemoglobin it contains.Materials1. Copper sulfate reagent2. Lancets3. Cotton4. AlcoholProcedure1. A single drop of patient's blood is placed in a solution of copper sulfatewith a specific gravity of 1.053 (Equivalent of density of blood with ahemoglobin value of 12.5 g/dl)2. Normal Test results occurs when drop of blood falls through the copsulfate solution without rising, indicating the blood has a >12.5 g/dlhemoglobin level.Precautions:> Do not mouth pipet the Drabkin's reagent. It contains cyanide and ispoisonous.Always use clean cuvettes.Ensure that the dilution is made correctly.Set the wavelength at 540nm.> Mix the blood ten to twenty times before sampling.> Do not squeeze the finger hard at the capillary puncture site.ROUTINE Hematology LabORATORYSTUDENT MANUAL ; Volume 1CALCULATIONS:1 Given the following values, use the absorbance formula to find thehemoglobin concentration of the unknown.As =0.600Au =0.300Cs =20g/dl2. Convert 12.8 g Hb into percent Hb

Blood is a biological fluid that flows within the blood vessels throughout the body. It is composed…

Answered: Given the following values, use the…

Given the following values, use the absorbance formula to find the hemoglobin concentration of the unknown. As =0.600 Au =0.300 Cs =20g/dl

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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