Time point (min)Absorbance of culture at 660nmApproximate cell concentrationApproximate # cells in 1mL extract00.2981.49 x 108 cells/mL1.49 x 108 cells100.3161.58 x 108 cells/mL1.58 x 108 cells200.3741.87 x 108 cells/mL1.87 x 108 cells300.4292.145 x 108 cells/mL2.145 x 108 cells400.5122.56 x 108 cells/mL2.56 x 108 cells500.5442.72 x 108 cells/mL2.72 x 108 cells600.6073.035 x 108 cells/mL3.035 x 108 cellsa. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.

The growth curve of a bacterial growth consists of four phases namely lag phase, log or exponential…

Answered: Time point (min) Absorbance of culture…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Question

Time point (min)	Absorbance of culture at 660nm	Approximate cell concentration	Approximate # cells in 1mL extract
0	0.298	1.49 x 10⁸ cells/mL	1.49 x 10⁸ cells
10	0.316	1.58 x 10⁸ cells/mL	1.58 x 10⁸ cells
20	0.374	1.87 x 10⁸ cells/mL	1.87 x 10⁸ cells
30	0.429	2.145 x 10⁸ cells/mL	2.145 x 10⁸ cells
40	0.512	2.56 x 10⁸ cells/mL	2.56 x 10⁸ cells
50	0.544	2.72 x 10⁸ cells/mL	2.72 x 10⁸ cells
60	0.607	3.035 x 10⁸ cells/mL	3.035 x 10⁸ cells

a. Using these data, prepare a growth curve of this strain of
Escherichia coli (E. coli).
b. Estimate the doubling time for this strain of E. Coli. Clearly show
how you estimated this value from the empirical data presented.

Expert Solution

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions