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Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Sulfur Indole Motility (SIM) Medium

H2S produced, color and +/-: ______________________________

Indole present/Tryptophan hydrolysis, color and +/-: ___________________________

Motile or non-motile: _____________________________

### SIM Deep Test: The image displays a test tube containing a SIM (Sulfide, Indole, Motility) medium, which is used in microbiology to detect hydrogen sulfide production, indole production, and bacterial motility. **Explanation of the SIM Deep Test:** 1. **Hydrogen Sulfide Production**: - **Observation**: If the medium turns black, it indicates that the bacterium produces hydrogen sulfide (H2S). - **Interpretation**: The black precipitate is a result of H2S reacting with iron salts in the medium to form ferric sulfide. 2. **Indole Production**: - **Test Procedure**: Following incubation, Kovac’s reagent is added to the medium. - **Observation**: A positive indole test is indicated by a red layer forming on the top of the medium after adding Kovac’s reagent. - **Interpretation**: The red color indicates the presence of indole, which is produced from the amino acid tryptophan by the enzyme tryptophanase. 3. **Motility**: - **Observation**: Motility is assessed by the diffuse growth radiating from the stab line of inoculation. - **Interpretation**: If the microorganism is motile, it will move away from the stab line and produce a cloudy appearance throughout the medium. Non-motile organisms will only grow along the stab line. In this image, the medium appears dark, potentially indicating the reduction of iron salts to a black precipitate (positive for hydrogen sulfide production). Further testing such as the addition of Kovac’s reagent would be needed to determine indole production. The motility can be assessed based on the diffusion of the organism away from the stab line in the medium. Ensure proper laboratory procedures and controls are maintained when performing this test for accurate results.
Transcribed Image Text:### SIM Deep Test: The image displays a test tube containing a SIM (Sulfide, Indole, Motility) medium, which is used in microbiology to detect hydrogen sulfide production, indole production, and bacterial motility. **Explanation of the SIM Deep Test:** 1. **Hydrogen Sulfide Production**: - **Observation**: If the medium turns black, it indicates that the bacterium produces hydrogen sulfide (H2S). - **Interpretation**: The black precipitate is a result of H2S reacting with iron salts in the medium to form ferric sulfide. 2. **Indole Production**: - **Test Procedure**: Following incubation, Kovac’s reagent is added to the medium. - **Observation**: A positive indole test is indicated by a red layer forming on the top of the medium after adding Kovac’s reagent. - **Interpretation**: The red color indicates the presence of indole, which is produced from the amino acid tryptophan by the enzyme tryptophanase. 3. **Motility**: - **Observation**: Motility is assessed by the diffuse growth radiating from the stab line of inoculation. - **Interpretation**: If the microorganism is motile, it will move away from the stab line and produce a cloudy appearance throughout the medium. Non-motile organisms will only grow along the stab line. In this image, the medium appears dark, potentially indicating the reduction of iron salts to a black precipitate (positive for hydrogen sulfide production). Further testing such as the addition of Kovac’s reagent would be needed to determine indole production. The motility can be assessed based on the diffusion of the organism away from the stab line in the medium. Ensure proper laboratory procedures and controls are maintained when performing this test for accurate results.
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