ext/map/ aining Lab Report Place the descriptions into the correct boxes to test your understanding of how errors would affect the Gram stain resu You did not heat fix the smear correctly. You forgot the crystal violet step. You forgot the Gram's lodine You applied the decolorizer for too long. You forgot to apply safranin. You forgot the decolorization step. Reset Gram-negative organisms might incorrectly stain purple. Gram-positive organisms might incorrectly stain pink Saved Gram-negative organisms might not be visible. You may not be able to view any organisms on the slide.

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: Why is Iodine used in the Gram Staining procedure?

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: What are the principle and basic concepts of DIFFERENTIAL staining?

A: The simple dye is used in the technique of simple staining to highlight only the specific structures…

Q: How reliable is PCR to detect microorganisms in water samples?

A: Polymerase chain reaction (PCR) is a technique discovered by Kary Mullis, which replicates DNA…

Q: Did each handwashing treatment result in similar types of organisms growing on the plates? (look at…

A: The science of tiny creatures' biology includes viruses, bacterium, algal, fungus, slime moulds, and…

Q: Why can we use drops instead of concentration to graph the data? What do we have to do…

A: concentration:  (1) The quantity of a sub-component (particularly a solute) in a solution. (2) The…

Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: In four steps, describe the simple staining procedure.

A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: How do you know if you Azotobacter bacteria isolation was successful? Could you please list all…

A: Azotobacter is a genus of free-living, nitrogen-fixing bacteria that are found in soil and water.…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: what is the gram staining procedure for e-coli

A: Gram staining is the laboratory technique which is used to differentiate between the morphology of…

Q: Why is it necessary to perform a negative stain to visualize capsules?

A: Introduction: Negative staining: This uses an acidic stain. The results yield a clear cell with a…

Q: What is the purpose of staining bacteria? What is the purpose of collecting bacteria from different…

A: The study regarding unidentified bacteria is explained to the reader in this part, along with its…

Q: List 3 species of bacteria that can spoil milk and indicate how they react to the gram staining…

A: Spoilage of milk and milk products results from growth of fermentative bacteria when storage…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…

A: The last two years have been extremely difficult because you can still learn and understand the…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.