Below is a trace from an analytical gel-filtration column upon which a single sample containing a mixture of two proteins was loaded. - 230nm 800 MicA:ClpC 600 - 400 - ClpC 200 - MicA 20 40 60 80 100 120 140 Volume (mL) Normalized absorbance mAU

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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What is happening in this trace? What do the peaks represent? Why is absorbance measured at 230 nm? 

Below is a trace from an analytical gel-filtration column upon
which a single sample containing a mixture of two proteins was
loaded.
800
- 230nm
MicA:ClpC
600 -
400
Clpc
200
MicA
20
40
60
80
100
120
140
Volume (mL)
Normalized absorbance mAU
Transcribed Image Text:Below is a trace from an analytical gel-filtration column upon which a single sample containing a mixture of two proteins was loaded. 800 - 230nm MicA:ClpC 600 - 400 Clpc 200 MicA 20 40 60 80 100 120 140 Volume (mL) Normalized absorbance mAU
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